Monolithic capillary columns based on methacryl-substituted polyhedral oligomeric silsesquioxane and sulfobetaine methacrylates synthesized in the wide-bore capillary: Evaluation and application in liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00618287" target="_blank" >RIV/68081715:_____/25:00618287 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0365130" target="_blank" >https://hdl.handle.net/11104/0365130</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2025.113239" target="_blank" >10.1016/j.microc.2025.113239</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monolithic capillary columns based on methacryl-substituted polyhedral oligomeric silsesquioxane and sulfobetaine methacrylates synthesized in the wide-bore capillary: Evaluation and application in liquid chromatography

Popis výsledku v původním jazyce

The protocol for preparation of methacryl-substituted polyhedral oligomeric silsesquioxane-based monolithic stationary phases containing zwitterionic sulfobetaine monomers in 450 µm i.d. fused silica capillaries is presented. The monolithic columns were evaluated by scanning electron microscopy, inverse size-exclusion chromatography, and isocratic HPLC separation of model compounds. Nature of zwitterionic sulfobetaine monomers has distinct impact on the structure of the final monolithic material as well as on its polarity and separation ability. The columns are suitable for multimodal chromatography as confirmed by their capability to separate compounds over a wide range of polarities (alkylbenzenes, phenolics, aromatic carboxylic acids, a mixture of purine-, pyrimidine-bases, nucleosides, and 2-deoxynucleosides). The critical mobile phase composition for the transition from hydrophilic interaction liquid chromatography to reversed-phase mode is around 84 % of acetonitrile which confirms the prevailing non-polar nature of formed monoliths. The proposed preparation protocol allowed preparation of stable columns whose efficiency reached the minimal height of the theoretical plate 6–10 µm which decreased by less than 12 % during the two months of everyday use.

Název v anglickém jazyce

Monolithic capillary columns based on methacryl-substituted polyhedral oligomeric silsesquioxane and sulfobetaine methacrylates synthesized in the wide-bore capillary: Evaluation and application in liquid chromatography

Popis výsledku anglicky

The protocol for preparation of methacryl-substituted polyhedral oligomeric silsesquioxane-based monolithic stationary phases containing zwitterionic sulfobetaine monomers in 450 µm i.d. fused silica capillaries is presented. The monolithic columns were evaluated by scanning electron microscopy, inverse size-exclusion chromatography, and isocratic HPLC separation of model compounds. Nature of zwitterionic sulfobetaine monomers has distinct impact on the structure of the final monolithic material as well as on its polarity and separation ability. The columns are suitable for multimodal chromatography as confirmed by their capability to separate compounds over a wide range of polarities (alkylbenzenes, phenolics, aromatic carboxylic acids, a mixture of purine-, pyrimidine-bases, nucleosides, and 2-deoxynucleosides). The critical mobile phase composition for the transition from hydrophilic interaction liquid chromatography to reversed-phase mode is around 84 % of acetonitrile which confirms the prevailing non-polar nature of formed monoliths. The proposed preparation protocol allowed preparation of stable columns whose efficiency reached the minimal height of the theoretical plate 6–10 µm which decreased by less than 12 % during the two months of everyday use.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA23-04703S" target="_blank" >GA23-04703S: Alternativní příprava kapilárních monolitických kolon z diskrétních částic s využitím superkritické vody</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

212

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

113239

Kód UT WoS článku

001442833000001

EID výsledku v databázi Scopus

2-s2.0-85219746037