3D-printed device for time- and cost-efficient sample preparation and DNA fractionation
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F25%3A00642593" target="_blank" >RIV/68081715:_____/25:00642593 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14310/25:00143554 RIV/00216208:11310/25:10509994
Výsledek na webu
<a href="https://hdl.handle.net/11104/0372452" target="_blank" >https://hdl.handle.net/11104/0372452</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2024.127461" target="_blank" >10.1016/j.talanta.2024.127461</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
3D-printed device for time- and cost-efficient sample preparation and DNA fractionation
Popis výsledku v původním jazyce
Direct detection of DNA in complex biological samples bears several challenges regarding the selectivity and sensitivity of analyses. Therefore, DNA pre-extraction from bio-fluids is an emerging tool in biologically related fields. Specifically, a newly developing family of liquid biopsy techniques using PCR detection of circulating tumor DNA from blood serum or blood plasma could be significantly improved by harnessing fast and highthroughput DNA sample preparation. To address these needs, a 3D-printed device and a method based on gel electrophoresis combined with electrodialysis for the time-, cost- and labor-efficient preparative separation of DNA fragments from blood was developed. The proposed system also successfully eliminated large DNA fragments from the samples. Recovery for short DNA fragments was reaching up to 80 %. The method was tested onnhuman genomic DNA and blood and blood serum spiked with DNA standards, and it significantly alleviated the signal of matrix DNA.
Název v anglickém jazyce
3D-printed device for time- and cost-efficient sample preparation and DNA fractionation
Popis výsledku anglicky
Direct detection of DNA in complex biological samples bears several challenges regarding the selectivity and sensitivity of analyses. Therefore, DNA pre-extraction from bio-fluids is an emerging tool in biologically related fields. Specifically, a newly developing family of liquid biopsy techniques using PCR detection of circulating tumor DNA from blood serum or blood plasma could be significantly improved by harnessing fast and highthroughput DNA sample preparation. To address these needs, a 3D-printed device and a method based on gel electrophoresis combined with electrodialysis for the time-, cost- and labor-efficient preparative separation of DNA fragments from blood was developed. The proposed system also successfully eliminated large DNA fragments from the samples. Recovery for short DNA fragments was reaching up to 80 %. The method was tested onnhuman genomic DNA and blood and blood serum spiked with DNA standards, and it significantly alleviated the signal of matrix DNA.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Talanta
ISSN
0039-9140
e-ISSN
1873-3573
Svazek periodika
286
Číslo periodika v rámci svazku
MAY
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
7
Strana od-do
127461
Kód UT WoS článku
001412356500001
EID výsledku v databázi Scopus
2-s2.0-85213531037