Raman microspectroscopy of algal lipid bodies: beta-carotene quantification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F12%3A00383732" target="_blank" >RIV/68081731:_____/12:00383732 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/12:00383732 RIV/60646594:_____/12:#0000008

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10811-011-9754-4" target="_blank" >http://dx.doi.org/10.1007/s10811-011-9754-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10811-011-9754-4" target="_blank" >10.1007/s10811-011-9754-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman microspectroscopy of algal lipid bodies: beta-carotene quantification

Popis výsledku v původním jazyce

Advanced optical instruments can serve for analysis and manipulation of individual living cells and their internal structures. We have used Raman microspectroscopic analysis for assessment of beta-carotene concentration in algal lipid bodies (LBs) in vivo. Some algae contain beta-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have devised a simple method to measure the concentration of beta-carotene in a mixtureof algal storage lipids from the ratio of their Raman vibrations. This finding may allow fast acquisition of beta-carotene concentration valuable, e.g., for Raman microspectroscopy assisted cell sorting for selection of the overproducing strains. Furthermore, we demonstrate that beta-carotene concentration can be proportional to LB volume and light intensity during the cultivation. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective

Název v anglickém jazyce

Raman microspectroscopy of algal lipid bodies: beta-carotene quantification

Popis výsledku anglicky

Advanced optical instruments can serve for analysis and manipulation of individual living cells and their internal structures. We have used Raman microspectroscopic analysis for assessment of beta-carotene concentration in algal lipid bodies (LBs) in vivo. Some algae contain beta-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have devised a simple method to measure the concentration of beta-carotene in a mixtureof algal storage lipids from the ratio of their Raman vibrations. This finding may allow fast acquisition of beta-carotene concentration valuable, e.g., for Raman microspectroscopy assisted cell sorting for selection of the overproducing strains. Furthermore, we demonstrate that beta-carotene concentration can be proportional to LB volume and light intensity during the cultivation. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Phycology

ISSN

0921-8971

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

541-546

Kód UT WoS článku

000304187100029

EID výsledku v databázi Scopus

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