Raman tweezers in microfluidic systems for analysis and sorting of living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00437742" target="_blank" >RIV/68081731:_____/14:00437742 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1117/12.2176047" target="_blank" >http://dx.doi.org/10.1117/12.2176047</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2176047" target="_blank" >10.1117/12.2176047</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman tweezers in microfluidic systems for analysis and sorting of living cells

Popis výsledku v původním jazyce

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical compositionof the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demon

Název v anglickém jazyce

Raman tweezers in microfluidic systems for analysis and sorting of living cells

Popis výsledku anglicky

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical compositionof the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demon

Druh

D - Stať ve sborníku

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441)

ISBN

9781628415568

ISSN

0277-786X

e-ISSN

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Počet stran výsledku

8

Strana od-do

"944107:1"-"8"

Název nakladatele

SPIE

Místo vydání

Bellingham

Místo konání akce

Jelenia Góra

Datum konání akce

8. 9. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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