Raman tweezers in microfluidic systems for analysis and sorting of living cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00434961" target="_blank" >RIV/68081731:_____/14:00434961 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1117/12.2040631" target="_blank" >http://dx.doi.org/10.1117/12.2040631</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2040631" target="_blank" >10.1117/12.2040631</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman tweezers in microfluidic systems for analysis and sorting of living cells

Popis výsledku v původním jazyce

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sort

Název v anglickém jazyce

Raman tweezers in microfluidic systems for analysis and sorting of living cells

Popis výsledku anglicky

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sort

Druh

D - Stať ve sborníku

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII (Proceedings of SPIE 8947)

ISBN

9780819498601

ISSN

1605-7422

e-ISSN

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Počet stran výsledku

9

Strana od-do

"89471M:1"-"9"

Název nakladatele

SPIE

Místo vydání

Bellingham

Místo konání akce

San Francisco

Datum konání akce

3. 2. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000336037200037