A new cryo high-vacuum transfer system for STEM and TEM

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A new cryo high-vacuum transfer system for STEM and TEM

Popis výsledku v původním jazyce

Every microscopist pursues the aim of examining their specimen in the native state. In the case of hydrated samples like filaments, cells, or labile systems like micelles, vesicles, lamellae and nano capsules, the embedding in amorphous ice is the meansof choice, Consequently, great efforts are being put into the optimization of cryo-preparation methods in order to utilize the full technical possibilities of techniques like cryo-electron tomography (CET) or quantitative scanning electron microscopy (q-STEM). Especially in the case of quantitative measurements, like the determination of mass or mass-thickness profiles, particular requirements are imposed on the purity of the specimen, However, prerequisites for high resolution investigations, e.g. cryo-electron microscopy (cryo-EM) of vitreous sections, are the avoidance of ice crystal damage by devitrification and the prevention of contamination of the frozen hydrated specimen during the entire handling. Therefore, the temperature of

Název v anglickém jazyce

A new cryo high-vacuum transfer system for STEM and TEM

Popis výsledku anglicky

Every microscopist pursues the aim of examining their specimen in the native state. In the case of hydrated samples like filaments, cells, or labile systems like micelles, vesicles, lamellae and nano capsules, the embedding in amorphous ice is the meansof choice, Consequently, great efforts are being put into the optimization of cryo-preparation methods in order to utilize the full technical possibilities of techniques like cryo-electron tomography (CET) or quantitative scanning electron microscopy (q-STEM). Especially in the case of quantitative measurements, like the determination of mass or mass-thickness profiles, particular requirements are imposed on the purity of the specimen, However, prerequisites for high resolution investigations, e.g. cryo-electron microscopy (cryo-EM) of vitreous sections, are the avoidance of ice crystal damage by devitrification and the prevention of contamination of the frozen hydrated specimen during the entire handling. Therefore, the temperature of

Druh

D - Stať ve sborníku

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

EMC 2012. Proceedings of the 15th European Microscopy Congress

ISBN

978-0-9502463-7-6

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

445-446

Název nakladatele

The Royal Microscopical Society

Místo vydání

Manchester

Místo konání akce

Manchester

Datum konání akce

16. 9. 2012

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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