A cryo high-vacuum shuttle for correlative cryogenic investigations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00434113" target="_blank" >RIV/68081731:_____/14:00434113 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A cryo high-vacuum shuttle for correlative cryogenic investigations

Popis výsledku v původním jazyce

The preservation of the native state is the key element in sample preparation. In the case of hydrated objects, embedding in vitreous (amorphous) ice and subsequent examination under cryogenic (cryo) conditions are the means of choice. Over the last years, cryogenic techniques such as cryo-electron microscopy (cryo-EM) or soft X-ray cryo-microscopy have become increasingly popular, as they provide a direct, unaltered view on the specimen. However, to provide a snapshot of the pristine architecture of the specimen, cryo techniques require constant cooling below the recrystallization temperature of 138°K and avoidance of any contamination. This has been proven to be particularly challenging in the case of correlative cryo investigations, since these methods include several transfer steps due to their extensive post-processing and complex workflow. In the past, several transfer concepts were introduced and they are now commercially available. However, these systems are limited either by n

Název v anglickém jazyce

A cryo high-vacuum shuttle for correlative cryogenic investigations

Popis výsledku anglicky

The preservation of the native state is the key element in sample preparation. In the case of hydrated objects, embedding in vitreous (amorphous) ice and subsequent examination under cryogenic (cryo) conditions are the means of choice. Over the last years, cryogenic techniques such as cryo-electron microscopy (cryo-EM) or soft X-ray cryo-microscopy have become increasingly popular, as they provide a direct, unaltered view on the specimen. However, to provide a snapshot of the pristine architecture of the specimen, cryo techniques require constant cooling below the recrystallization temperature of 138°K and avoidance of any contamination. This has been proven to be particularly challenging in the case of correlative cryo investigations, since these methods include several transfer steps due to their extensive post-processing and complex workflow. In the past, several transfer concepts were introduced and they are now commercially available. However, these systems are limited either by n

Druh

D - Stať ve sborníku

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-20012S" target="_blank" >GA14-20012S: Kvantitativní zobrazování v REM pomocí pružně rozptýlených elektronů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

18th International Microscopy Congres. Proceedings

ISBN

978-80-260-6720-7

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

Czechoslovak Microscopy Society

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

7. 9. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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