Processing a Biological Tissue from Cryo-SEM to Replica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465204" target="_blank" >RIV/68081731:_____/16:00465204 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S1431927616002038" target="_blank" >http://dx.doi.org/10.1017/S1431927616002038</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927616002038" target="_blank" >10.1017/S1431927616002038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Processing a Biological Tissue from Cryo-SEM to Replica

Popis výsledku v původním jazyce

Cryo-SEM is a technique suitable for observation of frozen biological samples. The cryo-preparation of tissue fragments without using cryo-protectants can destroy intracellular compartments and cytoplasm. On the other hand the use of cryoprotectants can prevent deeper etching of the medium and thus it often leads to more complicated detection of cell membranes, which are important for orientation in the sample during its observation. This can be managed by shadowing of the fractured structures by platinum and it may help to heighten the structures as is done in production of replicas which can give more detail at higher magnification. Here we report how the replicas can be prepared in the device ACE600 (Leica microsystems) equipped with the cryo-attachement and two guns for metal sputtering and carbon evaporation, and the combination of cryo-SEM and replicas.

Název v anglickém jazyce

Processing a Biological Tissue from Cryo-SEM to Replica

Popis výsledku anglicky

Cryo-SEM is a technique suitable for observation of frozen biological samples. The cryo-preparation of tissue fragments without using cryo-protectants can destroy intracellular compartments and cytoplasm. On the other hand the use of cryoprotectants can prevent deeper etching of the medium and thus it often leads to more complicated detection of cell membranes, which are important for orientation in the sample during its observation. This can be managed by shadowing of the fractured structures by platinum and it may help to heighten the structures as is done in production of replicas which can give more detail at higher magnification. Here we report how the replicas can be prepared in the device ACE600 (Leica microsystems) equipped with the cryo-attachement and two guns for metal sputtering and carbon evaporation, and the combination of cryo-SEM and replicas.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

S3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

236-237

Kód UT WoS článku

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EID výsledku v databázi Scopus

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