Is Sputtering Sufficient for Production of Replicas?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465205" target="_blank" >RIV/68081731:_____/16:00465205 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S1431927616001070" target="_blank" >http://dx.doi.org/10.1017/S1431927616001070</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927616001070" target="_blank" >10.1017/S1431927616001070</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Is Sputtering Sufficient for Production of Replicas?

Popis výsledku v původním jazyce

In the production of replicas there are many parameters which influence the authenticity and precision of the biological structures, e.g. manipulation with tissue pieces or cells, conditions of freeze-fracture technique or the process of replica preparation alone. The original protocols of replica processing have employed for many years devices such as BAF060 (BAL-TEC) that is equipped by cryo-stage and two guns for well specified directional metal and carbon evaporation. This technology is relatively expensive, in comparison to modern devices for cryo-preparation for electron microscopy like ACE600 (Leica microsystems). However, these modern devices are usually based on the metal sputtering in thenargon atmosphere and evaporation of carbon (e.g. ACE600 or the precursor MED020). Here we report on the protocol for production replicas using both types of devices (ACE600 and BAF060) andncomparison of the results.

Název v anglickém jazyce

Is Sputtering Sufficient for Production of Replicas?

Popis výsledku anglicky

In the production of replicas there are many parameters which influence the authenticity and precision of the biological structures, e.g. manipulation with tissue pieces or cells, conditions of freeze-fracture technique or the process of replica preparation alone. The original protocols of replica processing have employed for many years devices such as BAF060 (BAL-TEC) that is equipped by cryo-stage and two guns for well specified directional metal and carbon evaporation. This technology is relatively expensive, in comparison to modern devices for cryo-preparation for electron microscopy like ACE600 (Leica microsystems). However, these modern devices are usually based on the metal sputtering in thenargon atmosphere and evaporation of carbon (e.g. ACE600 or the precursor MED020). Here we report on the protocol for production replicas using both types of devices (ACE600 and BAF060) andncomparison of the results.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

S3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

44-45

Kód UT WoS článku

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EID výsledku v databázi Scopus

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