In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465246" target="_blank" >RIV/68081731:_____/16:00465246 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/9783527808465.EMC2016.5932/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/9783527808465.EMC2016.5932/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/9783527808465.EMC2016.5932" target="_blank" >10.1002/9783527808465.EMC2016.5932</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM

Popis výsledku v původním jazyce

Polyelectrolyte complex (PEC) capsules/beads are very important for biotechnological applications such as drug delivery and bacterial whole-cell biocatalyst development. The very beam-sensitive bio-polymer capsules are laboratory produced as a uniform with a controlled shape, size, membrane thickness, permeability and mechanical resistance. PEC capsules are very sensitive to any treatment and samples could be inspected in their fully native and functional state to prevent any misinterpretation. Characterization and study of PEC capsules properties is possible using thermodynamically stabile and fully wet state, precisely reached after very slow changing of conditions in the specimen chamber of ESEM. The morphological study using low current ESEM was already presented. The internal structure can be in solvent, semisolid or solid state, depend on capsule type and manufacturing process, nevertheless it was not described in its native state yet. Study of inner part as well as surface morphology of PEC capsules using classical SEM or cryo-SEM can be misleading due to requirement of dry resp. freeze sample. The aim of this work is in-situ study of internal structure of PEC capsules in fully wet state and demonstration of state of matter of PEC capsules core.

Název v anglickém jazyce

In Situ Study of Internal Structure of Spherical Polyelectrolyte Complex Capsules Using ESEM

Popis výsledku anglicky

Polyelectrolyte complex (PEC) capsules/beads are very important for biotechnological applications such as drug delivery and bacterial whole-cell biocatalyst development. The very beam-sensitive bio-polymer capsules are laboratory produced as a uniform with a controlled shape, size, membrane thickness, permeability and mechanical resistance. PEC capsules are very sensitive to any treatment and samples could be inspected in their fully native and functional state to prevent any misinterpretation. Characterization and study of PEC capsules properties is possible using thermodynamically stabile and fully wet state, precisely reached after very slow changing of conditions in the specimen chamber of ESEM. The morphological study using low current ESEM was already presented. The internal structure can be in solvent, semisolid or solid state, depend on capsule type and manufacturing process, nevertheless it was not described in its native state yet. Study of inner part as well as surface morphology of PEC capsules using classical SEM or cryo-SEM can be misleading due to requirement of dry resp. freeze sample. The aim of this work is in-situ study of internal structure of PEC capsules in fully wet state and demonstration of state of matter of PEC capsules core.

Druh

D - Stať ve sborníku

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

EMC2016. The 16th European Microscopy Congress. Proceedings

ISBN

9783527808465

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

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Název nakladatele

Wiley

Místo vydání

Oxford

Místo konání akce

Lyon

Datum konání akce

28. 8. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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