A versatile high-vacuum cryo transfer system for cryo microscopy and analytics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465341" target="_blank" >RIV/68081731:_____/16:00465341 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/9783527808465.EMC2016.6577" target="_blank" >http://dx.doi.org/10.1002/9783527808465.EMC2016.6577</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/9783527808465.EMC2016.6577" target="_blank" >10.1002/9783527808465.EMC2016.6577</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A versatile high-vacuum cryo transfer system for cryo microscopy and analytics

Popis výsledku v původním jazyce

The conservation of the native state during sample preparation is mandatory for a correct interpretation of any micrograph. Particularly for EM, the preservation of the pristine architecture is challenging. If not imaged in situ, two different strategies can be followed to prepare hydrated samples for electron microscopy: the conventional and the cryogenic routine. Conventional preparation protocols typically rely on the chemical fixation and staining of the sample material, whereby both steps are known to induce artefacts. The second preparation routine, introduced by Moor and Dubochet, circumvents chemical artefacts. The basic principle of every cryogenic (cryo) preparation protocol is the physical immobilization of the sample in a frozen-hydrated solid state, by vitrifying the sample within milliseconds. Due to the excellent structural preservation cryo imaging techniques have gained increasing popularity.

Název v anglickém jazyce

A versatile high-vacuum cryo transfer system for cryo microscopy and analytics

Popis výsledku anglicky

The conservation of the native state during sample preparation is mandatory for a correct interpretation of any micrograph. Particularly for EM, the preservation of the pristine architecture is challenging. If not imaged in situ, two different strategies can be followed to prepare hydrated samples for electron microscopy: the conventional and the cryogenic routine. Conventional preparation protocols typically rely on the chemical fixation and staining of the sample material, whereby both steps are known to induce artefacts. The second preparation routine, introduced by Moor and Dubochet, circumvents chemical artefacts. The basic principle of every cryogenic (cryo) preparation protocol is the physical immobilization of the sample in a frozen-hydrated solid state, by vitrifying the sample within milliseconds. Due to the excellent structural preservation cryo imaging techniques have gained increasing popularity.

Druh

D - Stať ve sborníku

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

<a href="/cs/project/GA14-20012S" target="_blank" >GA14-20012S: Kvantitativní zobrazování v REM pomocí pružně rozptýlených elektronů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

EMC2016. The 16th European Microscopy Congres. Proceedings

ISBN

9783527808465

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

412-413

Název nakladatele

Wiley

Místo vydání

Oxford

Místo konání akce

Lyon

Datum konání akce

28. 8. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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