Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00499922" target="_blank" >RIV/68081731:_____/18:00499922 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s41377-018-0111-0" target="_blank" >http://dx.doi.org/10.1038/s41377-018-0111-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41377-018-0111-0" target="_blank" >10.1038/s41377-018-0111-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Popis výsledku v původním jazyce

Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

Název v anglickém jazyce

Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber

Popis výsledku anglicky

Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

<a href="/cs/project/EF15_003%2F0000476" target="_blank" >EF15_003/0000476: Holografická endoskopie pro in vivo aplikace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Light-Science & Applications

ISSN

2047-7538

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000453577600002

EID výsledku v databázi Scopus

2-s2.0-85058859483