The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F18%3A00504760" target="_blank" >RIV/68081731:_____/18:00504760 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/18:00504760 RIV/61989592:15310/19:73596008

Výsledek na webu

<a href="http://hdl.handle.net/11104/0296319" target="_blank" >http://hdl.handle.net/11104/0296319</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/0269249X.2018.1545703" target="_blank" >10.1080/0269249X.2018.1545703</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo

Popis výsledku v původním jazyce

The Low Temperature Method for sample stabilization in environmental scanning electron microscopy (LTM for ESEM) is a promising tool for the observation of diatom assemblages in vivo. Use of the environmental scanning electron microscope, in comparison to the conventional scanning electron microscope, enables study of fresh material without any chemical pretreatment and conductive coating. The newly developed Low Temperature Method, introduced in this paper, offers higher resolution and better resistance of wet samples to radiation damage. We used natural epiphytic algal assemblages to image 3D structure of: i) biofilm/periphyton and its physical complexity, ii) diatoms with their extracellular mucilaginous secretions enabling cells to attach to the substrate, iii) diatom colony formation, and iv) intact diatom cells in contrast to separated empty valves observed in the conventional scanning electron microscope. This study demonstrates the potential of this new method for environmental scanning electron microscopy in diatom biology and ecology in comparison with other imaging methods.

Název v anglickém jazyce

The Low Temperature Method for Environmental Scanning Electron Microscopy – a New Method for Observation of Diatom Assemblages in vivo

Popis výsledku anglicky

The Low Temperature Method for sample stabilization in environmental scanning electron microscopy (LTM for ESEM) is a promising tool for the observation of diatom assemblages in vivo. Use of the environmental scanning electron microscope, in comparison to the conventional scanning electron microscope, enables study of fresh material without any chemical pretreatment and conductive coating. The newly developed Low Temperature Method, introduced in this paper, offers higher resolution and better resistance of wet samples to radiation damage. We used natural epiphytic algal assemblages to image 3D structure of: i) biofilm/periphyton and its physical complexity, ii) diatoms with their extracellular mucilaginous secretions enabling cells to attach to the substrate, iii) diatom colony formation, and iv) intact diatom cells in contrast to separated empty valves observed in the conventional scanning electron microscope. This study demonstrates the potential of this new method for environmental scanning electron microscopy in diatom biology and ecology in comparison with other imaging methods.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diatom Research

ISSN

0269-249X

e-ISSN

—

Svazek periodika

33

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

397-403

Kód UT WoS článku

000459800300008

EID výsledku v databázi Scopus

2-s2.0-85059693525