Micro-morphological Characterization of In-Vivo Diatoms Using ESEM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F17%3A00481040" target="_blank" >RIV/67985939:_____/17:00481040 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/17:00481040

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S1431927617007395" target="_blank" >http://dx.doi.org/10.1017/S1431927617007395</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927617007395" target="_blank" >10.1017/S1431927617007395</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micro-morphological Characterization of In-Vivo Diatoms Using ESEM

Popis výsledku v původním jazyce

Diatoms (Bacillariophyta) are one of the most distinctive and successful groups of unicellula photosynthetic algae. They are ecologically widespread inhabiting mainly fresh, brackish and marine waters, often dominant in given microhabitats and contribute about one fourth to the world’s productivity (in terms of carbon fixation). Diatoms are also the most species-rich algal group represented by 50 – 200 thousand species. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule which is composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating. This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of: 1) whole intact diatom cells, not only empty diatom frustules, 2) extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes), 3) whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating. Observation of these aquatic organisms in their native wet state is not yet common even if it allows study of diatoms adhered directly on the host plant. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method for the ESEM (LTM), ideally in a combination with advanced low noise detectors with very high detection efficiency.

Název v anglickém jazyce

Micro-morphological Characterization of In-Vivo Diatoms Using ESEM

Popis výsledku anglicky

Diatoms (Bacillariophyta) are one of the most distinctive and successful groups of unicellula photosynthetic algae. They are ecologically widespread inhabiting mainly fresh, brackish and marine waters, often dominant in given microhabitats and contribute about one fourth to the world’s productivity (in terms of carbon fixation). Diatoms are also the most species-rich algal group represented by 50 – 200 thousand species. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule which is composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating. This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of: 1) whole intact diatom cells, not only empty diatom frustules, 2) extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes), 3) whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating. Observation of these aquatic organisms in their native wet state is not yet common even if it allows study of diatoms adhered directly on the host plant. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method for the ESEM (LTM), ideally in a combination with advanced low noise detectors with very high detection efficiency.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

—

Svazek periodika

23

Číslo periodika v rámci svazku

S1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

1346-1347

Kód UT WoS článku

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EID výsledku v databázi Scopus

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