Study of Plant Waxes Using Low Temperature Method for ESEM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00465203" target="_blank" >RIV/68081731:_____/16:00465203 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10337586

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S1431927616006747" target="_blank" >http://dx.doi.org/10.1017/S1431927616006747</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927616006747" target="_blank" >10.1017/S1431927616006747</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of Plant Waxes Using Low Temperature Method for ESEM

Popis výsledku v původním jazyce

A possibility to study biological samples in their native state, or even living organisms, using an environmental scanning electron microscope (ESEM) is of great importance for biologists. Sputter-free, electrically non-conductive and wet biological samples in their living and native state are observed using ESEM under conditions of high pressure water vapors that ranges from units to thousands of Pa in the ESEM specimen chamber. One of unwanted consequences of the electron beam-gas interaction is the electrons scattering that results in beam spot defocusing followed by the decrease of the detected signalto-noise ratio. This can be compensated by the reduction of beam electrons gas path length and scanning speed or by the increasing of the beam energy. The latter two parameters fundamentally enhance radiation damage of sensitive and soft native samples and represent a limiting factor for their observation using ESEM. This problem can be overcome by the use of special methods like Low Temperature Method for the ESEM (LTM), ideally in a combination with advanced low noise detectors with very high detection efficiencies.

Název v anglickém jazyce

Study of Plant Waxes Using Low Temperature Method for ESEM

Popis výsledku anglicky

A possibility to study biological samples in their native state, or even living organisms, using an environmental scanning electron microscope (ESEM) is of great importance for biologists. Sputter-free, electrically non-conductive and wet biological samples in their living and native state are observed using ESEM under conditions of high pressure water vapors that ranges from units to thousands of Pa in the ESEM specimen chamber. One of unwanted consequences of the electron beam-gas interaction is the electrons scattering that results in beam spot defocusing followed by the decrease of the detected signalto-noise ratio. This can be compensated by the reduction of beam electrons gas path length and scanning speed or by the increasing of the beam energy. The latter two parameters fundamentally enhance radiation damage of sensitive and soft native samples and represent a limiting factor for their observation using ESEM. This problem can be overcome by the use of special methods like Low Temperature Method for the ESEM (LTM), ideally in a combination with advanced low noise detectors with very high detection efficiencies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

S3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

1180-1181

Kód UT WoS článku

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EID výsledku v databázi Scopus

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