Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F20%3A00534715" target="_blank" >RIV/68081731:_____/20:00534715 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/20:00114355 RIV/00216305:26310/20:PU137478 RIV/00216208:11310/20:10414371 RIV/00159816:_____/20:00073209

Výsledek na webu

<a href="https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963" target="_blank" >https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c01963</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.analchem.0c01963" target="_blank" >10.1021/acs.analchem.0c01963</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Popis výsledku v původním jazyce

Bacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.

Název v anglickém jazyce

Analysis of Bacteriophage-Host Interaction by Raman Tweezers

Popis výsledku anglicky

Bacteriophages, or phages for short, are viruses that replicate in bacteria. The therapeutic and biotechnological potential of phages and their lytic enzymes is of interest for their ability to selectively destroy pathogenic bacteria, including antibiotic-resistant strains. Introduction of phage preparations into medicine, biotechnology, and food industry requires a thorough characterization of phage-host interaction on a molecular level. We employed Raman tweezers to analyze the phage-host interaction of Staphylococcus aureus strain FS159 with a virulent phage JK2 (=812K1/420) of the Myoviridae family and a temperate phage 80 alpha of the Siphoviridae family. We analyzed the timeline of phage-induced molecular changes in infected host cells. We reliably detected the presence of replicating phages in bacterial cells within 5 min after infection. Our results lay the foundations for building a Raman-based diagnostic instrument capable of real-time, in vivo, in situ, nondestructive characterization of the phage-host relationship on the level of individual cells, which has the potential of importantly contributing to the development of phage therapy and enzybiotics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Chemistry

ISSN

0003-2700

e-ISSN

—

Svazek periodika

92

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

12304-12311

Kód UT WoS článku

000572832900029

EID výsledku v databázi Scopus

2-s2.0-85094979024