Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F20%3A00535569" target="_blank" >RIV/68081731:_____/20:00535569 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11359/1135907/Coherent-anti-Stokes-Raman-scattering-microscopy-through-a-multimode-fiber/10.1117/12.2555080.short" target="_blank" >https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11359/1135907/Coherent-anti-Stokes-Raman-scattering-microscopy-through-a-multimode-fiber/10.1117/12.2555080.short</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2555080" target="_blank" >10.1117/12.2555080</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Popis výsledku v původním jazyce

Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Název v anglickém jazyce

Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Popis výsledku anglicky

Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biomedical Spectroscopy, Microscopy, and Imaging 2020. Proceedings of SPIE

ISBN

978-151063490-9

ISSN

0277-786X

e-ISSN

—

Počet stran výsledku

9

Strana od-do

162267

Název nakladatele

SPIE

Místo vydání

Bellingham

Místo konání akce

France

Datum konání akce

6. 4. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000674749600004