Polarization-resolved second-harmonic generation imaging through a multimode fiber

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F21%3A00544708" target="_blank" >RIV/68081731:_____/21:00544708 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.osapublishing.org/optica/fulltext.cfm?uri=optica-8-8-1065&id=453930" target="_blank" >https://www.osapublishing.org/optica/fulltext.cfm?uri=optica-8-8-1065&id=453930</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1364/OPTICA.430295" target="_blank" >10.1364/OPTICA.430295</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Polarization-resolved second-harmonic generation imaging through a multimode fiber

Popis výsledku v původním jazyce

Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such a fiber endoscope. We simultaneously fully control the excitation polarization state and the spatial distribution of the light at the fiber tip, and we use this to implement polarization-resolved SHG imaging, which allows imaging and identification of structural proteins such as collagen and myosin.We image mouse tail tendon and heart tissue, employing the endoscope at depths up to 1 mm, demonstrating that we can differentiate these structural proteins. This method has the potential for enabling instant and in situ diagnosis of tumors and fibrotic conditions insensitive tissue with minimal damage.

Název v anglickém jazyce

Polarization-resolved second-harmonic generation imaging through a multimode fiber

Popis výsledku anglicky

Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such a fiber endoscope. We simultaneously fully control the excitation polarization state and the spatial distribution of the light at the fiber tip, and we use this to implement polarization-resolved SHG imaging, which allows imaging and identification of structural proteins such as collagen and myosin.We image mouse tail tendon and heart tissue, employing the endoscope at depths up to 1 mm, demonstrating that we can differentiate these structural proteins. This method has the potential for enabling instant and in situ diagnosis of tumors and fibrotic conditions insensitive tissue with minimal damage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Optica

ISSN

2334-2536

e-ISSN

2334-2536

Svazek periodika

8

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1065-1074

Kód UT WoS článku

000687747700005

EID výsledku v databázi Scopus

2-s2.0-85111981587