Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F21%3A00549488" target="_blank" >RIV/68081731:_____/21:00549488 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/21:00074767 RIV/00216224:14110/21:00121360

Výsledek na webu

<a href="https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1" target="_blank" >https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1140/epjp/s13360-021-01152-1" target="_blank" >10.1140/epjp/s13360-021-01152-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Popis výsledku v původním jazyce

Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.

Název v anglickém jazyce

Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Popis výsledku anglicky

Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Physical Journal Plus

ISSN

2190-5444

e-ISSN

2190-5444

Svazek periodika

136

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

233

Kód UT WoS článku

000621061800003

EID výsledku v databázi Scopus

2-s2.0-85101346119