Hybrid multimode - multicore fibre based holographic endoscope for deep-tissue neurophotonics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F22%3A00564722" target="_blank" >RIV/68081731:_____/22:00564722 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.light-am.com/article/doi/10.37188/lam.2022.029" target="_blank" >https://www.light-am.com/article/doi/10.37188/lam.2022.029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37188/lam.2022.029" target="_blank" >10.37188/lam.2022.029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hybrid multimode - multicore fibre based holographic endoscope for deep-tissue neurophotonics

Popis výsledku v původním jazyce

n-vivo microendoscopy in animal models became a groundbreaking technique in neuroscience that rapidly expands our understanding of the brain. Emerging hair-thin endoscopes based on multimode fibres are now opening up the prospect of ultra-minimally invasive neuroimaging of deeply located brain structures. Complementing these advancements with methods of functional imaging and optogenetics, as well as extending its applicability to awake and motile animals constitute the most pressing challenges for this technology. Here we demonstrate a novel fibre design capable of both, high-resolution imaging in immobilised animals and bending-resilient optical addressing of neurons in motile animals. The optimised refractive index profile and the probe structure allowed reaching a spatial resolution of 2 μm across a 230 μm field of view for the initial layout of the fibre. Simultaneously, the fibre exhibits negligible cross-talk between individual inner-cores during fibre deformation. This work provides a technological solution for imaging-assisted spatially selective photo-activation and activity monitoring in awake and freely moving animal models.

Název v anglickém jazyce

Hybrid multimode - multicore fibre based holographic endoscope for deep-tissue neurophotonics

Popis výsledku anglicky

n-vivo microendoscopy in animal models became a groundbreaking technique in neuroscience that rapidly expands our understanding of the brain. Emerging hair-thin endoscopes based on multimode fibres are now opening up the prospect of ultra-minimally invasive neuroimaging of deeply located brain structures. Complementing these advancements with methods of functional imaging and optogenetics, as well as extending its applicability to awake and motile animals constitute the most pressing challenges for this technology. Here we demonstrate a novel fibre design capable of both, high-resolution imaging in immobilised animals and bending-resilient optical addressing of neurons in motile animals. The optimised refractive index profile and the probe structure allowed reaching a spatial resolution of 2 μm across a 230 μm field of view for the initial layout of the fibre. Simultaneously, the fibre exhibits negligible cross-talk between individual inner-cores during fibre deformation. This work provides a technological solution for imaging-assisted spatially selective photo-activation and activity monitoring in awake and freely moving animal models.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

<a href="/cs/project/EF15_003%2F0000476" target="_blank" >EF15_003/0000476: Holografická endoskopie pro in vivo aplikace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Light: Advanced Manufacturing

ISSN

2689-9620

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CN - Čínská lidová republika

Počet stran výsledku

9

Strana od-do

29

Kód UT WoS článku

001350219300001

EID výsledku v databázi Scopus

2-s2.0-85148611611