Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F22%3A00565445" target="_blank" >RIV/68081731:_____/22:00565445 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/22:00077925 RIV/00216224:14110/22:00125725

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcimb.2022.866463" target="_blank" >10.3389/fcimb.2022.866463</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings

Popis výsledku v původním jazyce

Rapid and accurate identification of pathogens causing infections is one of the biggest challenges in medicine. Timely identification of causative agents and their antimicrobial resistance profile can significantly improve the management of infection, lower costs for healthcare, mitigate ever-growing antimicrobial resistance and in many cases, save lives. Raman spectroscopy was shown to be a useful-quick, non-invasive, and non-destructivetool for identifying microbes from solid and liquid media. Modifications of Raman spectroscopy and/or pretreatment of samples allow single-cell analyses and identification of microbes from various samples. It was shown that those non-culture-based approaches could also detect antimicrobial resistance. Moreover, recent studies suggest that a combination of Raman spectroscopy with optical tweezers has the potential to identify microbes directly from human body fluids. This review aims to summarize recent advances in non-culture-based approaches of identification of microbes and their virulence factors, including antimicrobial resistance, using methods based on Raman spectroscopy in the context of possible use in the future point-of-care diagnostic process.

Název v anglickém jazyce

Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings

Popis výsledku anglicky

Rapid and accurate identification of pathogens causing infections is one of the biggest challenges in medicine. Timely identification of causative agents and their antimicrobial resistance profile can significantly improve the management of infection, lower costs for healthcare, mitigate ever-growing antimicrobial resistance and in many cases, save lives. Raman spectroscopy was shown to be a useful-quick, non-invasive, and non-destructivetool for identifying microbes from solid and liquid media. Modifications of Raman spectroscopy and/or pretreatment of samples allow single-cell analyses and identification of microbes from various samples. It was shown that those non-culture-based approaches could also detect antimicrobial resistance. Moreover, recent studies suggest that a combination of Raman spectroscopy with optical tweezers has the potential to identify microbes directly from human body fluids. This review aims to summarize recent advances in non-culture-based approaches of identification of microbes and their virulence factors, including antimicrobial resistance, using methods based on Raman spectroscopy in the context of possible use in the future point-of-care diagnostic process.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

<a href="/cs/project/NU21-05-00341" target="_blank" >NU21-05-00341: Pokročilé metody detekce patogenních mikroorganizmů a hodnocení účinku antimikrobiálních látek Ramanovou spektroskopií a dalšími metodami na úrovni molekul v optofluidních systémech</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Cellular and Infection Microbiology

ISSN

2235-2988

e-ISSN

2235-2988

Svazek periodika

12

Číslo periodika v rámci svazku

22 April

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

866463

Kód UT WoS článku

000810475900001

EID výsledku v databázi Scopus

2-s2.0-85129652774