Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F22%3A00553555" target="_blank" >RIV/68081731:_____/22:00553555 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/22:00076328 RIV/00216224:14110/22:00125080 RIV/62157124:16170/22:43880478

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0003267021011181" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0003267021011181</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2021.339292" target="_blank" >10.1016/j.aca.2021.339292</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections

Popis výsledku v původním jazyce

Urinary tract infections belong to the most common infections in the world. Besides communityacquired infections, nosocomial infections pose a high risk especially for patients having indwelling catheters, undergoing urological surgeries or staying at hospital for prolonged time. They can be often complicated by antimicrobial resistance and/or biofilm formation. Therefore, a rapid diagnostic tool enabling timely identification of a causative agent and its susceptibility to antimicrobials is a need. Raman spectroscopy appears to be a suitable method that allows rapid differentiation among microbes and provides a space for further analyses, such as determination of capability of biofilm formation or antimicrobial susceptibility/resistance in tested strains. Our work here presents a possibility to differ among most common microbes causing urinary tract infections (belonging to 20 species). We tested 254 strains directly from colonies grown on Mueller-Hinton agar plates. The results show that it is possible to distinguish among the tested species using Raman spectroscopy, which proves its great potential for future use in clinical diagnostics. Moreover, we present here a pilot study of a real-time analysis and identification (in less than 10 min) of single microbial cells directly in urine employing optical tweezers combined with Raman spectroscopy.

Název v anglickém jazyce

Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections

Popis výsledku anglicky

Urinary tract infections belong to the most common infections in the world. Besides communityacquired infections, nosocomial infections pose a high risk especially for patients having indwelling catheters, undergoing urological surgeries or staying at hospital for prolonged time. They can be often complicated by antimicrobial resistance and/or biofilm formation. Therefore, a rapid diagnostic tool enabling timely identification of a causative agent and its susceptibility to antimicrobials is a need. Raman spectroscopy appears to be a suitable method that allows rapid differentiation among microbes and provides a space for further analyses, such as determination of capability of biofilm formation or antimicrobial susceptibility/resistance in tested strains. Our work here presents a possibility to differ among most common microbes causing urinary tract infections (belonging to 20 species). We tested 254 strains directly from colonies grown on Mueller-Hinton agar plates. The results show that it is possible to distinguish among the tested species using Raman spectroscopy, which proves its great potential for future use in clinical diagnostics. Moreover, we present here a pilot study of a real-time analysis and identification (in less than 10 min) of single microbial cells directly in urine employing optical tweezers combined with Raman spectroscopy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

1873-4324

Svazek periodika

1191

Číslo periodika v rámci svazku

25 January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

339292

Kód UT WoS článku

000744469700002

EID výsledku v databázi Scopus

2-s2.0-85120916888