Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F23%3A00577394" target="_blank" >RIV/68081731:_____/23:00577394 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14110/23:00131818 RIV/00159816:_____/23:00079451
Výsledek na webu
<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2023.1272734" target="_blank" >10.3389/fmicb.2023.1272734</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis
Popis výsledku v původním jazyce
Introduction Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity.Methods In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress.Results We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (-20 degrees C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains.Conclusion The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design.
Název v anglickém jazyce
Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis
Popis výsledku anglicky
Introduction Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity.Methods In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress.Results We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (-20 degrees C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains.Conclusion The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/NU21-05-00341" target="_blank" >NU21-05-00341: Pokročilé metody detekce patogenních mikroorganizmů a hodnocení účinku antimikrobiálních látek Ramanovou spektroskopií a dalšími metodami na úrovni molekul v optofluidních systémech</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Frontiers in Microbiology
ISSN
1664-302X
e-ISSN
1664-302X
Svazek periodika
14
Číslo periodika v rámci svazku
29 September
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
12
Strana od-do
1272734
Kód UT WoS článku
001085828700001
EID výsledku v databázi Scopus
2-s2.0-85174573280