Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00638398" target="_blank" >RIV/68081731:_____/25:00638398 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/25:00141772 RIV/00159816:_____/25:00082346

Výsledek na webu

<a href="https://www.mdpi.com/1424-8220/25/15/4629" target="_blank" >https://www.mdpi.com/1424-8220/25/15/4629</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s25154629" target="_blank" >10.3390/s25154629</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

Popis výsledku v původním jazyce

Highlights: What are the main findings? Adenine was detected as a stress marker in five bacterial strains. Stable Au and Ag NPs enabled SERS-based analysis of secreted bacterial metabolites. What is the implication of the main findings? Label-free SERS detects metabolic stress responses in bacteria cost-effectively. Gold NPs enable long-term, reproducible Raman-based bacterial stress detection. Abstract: This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor-demineralized water-inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.

Název v anglickém jazyce

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions

Popis výsledku anglicky

Highlights: What are the main findings? Adenine was detected as a stress marker in five bacterial strains. Stable Au and Ag NPs enabled SERS-based analysis of secreted bacterial metabolites. What is the implication of the main findings? Label-free SERS detects metabolic stress responses in bacteria cost-effectively. Gold NPs enable long-term, reproducible Raman-based bacterial stress detection. Abstract: This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor-demineralized water-inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

<a href="/cs/project/LM2023050" target="_blank" >LM2023050: Národní infrastruktura pro biologické a medicínské zobrazování</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors

ISSN

1424-8220

e-ISSN

1424-8220

Svazek periodika

25

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

4629

Kód UT WoS článku

001549756200001

EID výsledku v databázi Scopus

2-s2.0-105013297439