Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F22%3A00359895" target="_blank" >RIV/68407700:21230/22:00359895 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1021/acsanm.2c02571" target="_blank" >https://doi.org/10.1021/acsanm.2c02571</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsanm.2c02571" target="_blank" >10.1021/acsanm.2c02571</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Popis výsledku v původním jazyce

Surface-enhanced Raman spectroscopy (SERS) is a promising analytical technique for fast and accurate disease detection due to its attractive features. However, realizing label-free direct detection is still challenging as most probes have extremely low Raman cross sections and little affinity to SERS substrates. Disappointingly, SERS spectra of most bacteria and other biological samples look similar as the differences in their molecular compositions are subtle and not detectable. Hence, the fabrication of highly enhancing plasmonic nanoparticles with excellent uniformity is demanded. Moreover, as SERS substrates are not reusable, cost-effective and simplistic fabrication methods are critical. Here, we report a facile approach to synthesizing Ag nanoparticle array on Cu-foil in less than 3 min, using only Cu-foil, silver nitrate, and hydroquinone. We employed the idea of galvanic replacement in combination with a seed mediated particle-growth approach. The label-free bacterial detection has shown that our Cu/Ag nanoparticle substrate is superior to highly acclaimed Ag nanocubes. Creating strong second and third-generation SERS hot-spots through cooperative interaction of homo-(Ag-Ag) and heterogeneous (Ag-Cu) surfaces contributes mainly to the observed excellent enhancement. Interestingly, direct liquid bacteria sample analysis showed a 6-fold higher detection sensitivity than completely dried samples. We believe that our approach will offer remarkable advantages and change how SERS substrates are prepared and conducted in SERS-based bacterial detection.

Název v anglickém jazyce

Cu/Ag Nanoparticle-Based Surface-Enhanced Raman Scattering Substrates for Label-Free Bacterial Detection

Popis výsledku anglicky

Surface-enhanced Raman spectroscopy (SERS) is a promising analytical technique for fast and accurate disease detection due to its attractive features. However, realizing label-free direct detection is still challenging as most probes have extremely low Raman cross sections and little affinity to SERS substrates. Disappointingly, SERS spectra of most bacteria and other biological samples look similar as the differences in their molecular compositions are subtle and not detectable. Hence, the fabrication of highly enhancing plasmonic nanoparticles with excellent uniformity is demanded. Moreover, as SERS substrates are not reusable, cost-effective and simplistic fabrication methods are critical. Here, we report a facile approach to synthesizing Ag nanoparticle array on Cu-foil in less than 3 min, using only Cu-foil, silver nitrate, and hydroquinone. We employed the idea of galvanic replacement in combination with a seed mediated particle-growth approach. The label-free bacterial detection has shown that our Cu/Ag nanoparticle substrate is superior to highly acclaimed Ag nanocubes. Creating strong second and third-generation SERS hot-spots through cooperative interaction of homo-(Ag-Ag) and heterogeneous (Ag-Cu) surfaces contributes mainly to the observed excellent enhancement. Interestingly, direct liquid bacteria sample analysis showed a 6-fold higher detection sensitivity than completely dried samples. We believe that our approach will offer remarkable advantages and change how SERS substrates are prepared and conducted in SERS-based bacterial detection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Applied Nano Materials

ISSN

2574-0970

e-ISSN

2574-0970

Svazek periodika

5

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

11567-11576

Kód UT WoS článku

000841239500001

EID výsledku v databázi Scopus

2-s2.0-85136472024