Effect of the salivary gland and midgut extracts from Ixodes ricinus and Dermacentor reticulatus (Acari : Ixodidae) on the growth of Borrelia garinii in vitro.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F03%3A67030034" target="_blank" >RIV/68081766:_____/03:67030034 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of the salivary gland and midgut extracts from Ixodes ricinus and Dermacentor reticulatus (Acari : Ixodidae) on the growth of Borrelia garinii in vitro.

Popis výsledku v původním jazyce

In our study we examined the effect of salivary gland extract (SGE) and midgut extract (MGE) from Ixodes ricinus and Dermacentor reticulatus on the growth, motility and morphology of the B. garinii spirochete in vitro. Concentration of motile spirochetes(the number of motile cells/ml of medium) was determined at specific intervals using darkfield microscopy. In I. ricinus, the concentration of motile spirochetes increased significantly between the days 2 and 11 post inoculation (p.i.) with both SGE andMGE compared to control (C). In D. reticulatus, a significant increase in concentration of motile spirochetes was only detected with SGE (compared to control) on the day 5 p.i., while a marked decrease in concentration of motile spirochetes was observedon day 9 p.i. due to MGE effect, and on day 12 p.i. due to the effect of both extracts compared to control. The effect of SGE and MGE on the growth of B. garinii in vitro differed between the two ticks species tested: while the extracts

Název v anglickém jazyce

Effect of the salivary gland and midgut extracts from Ixodes ricinus and Dermacentor reticulatus (Acari : Ixodidae) on the growth of Borrelia garinii in vitro.

Popis výsledku anglicky

In our study we examined the effect of salivary gland extract (SGE) and midgut extract (MGE) from Ixodes ricinus and Dermacentor reticulatus on the growth, motility and morphology of the B. garinii spirochete in vitro. Concentration of motile spirochetes(the number of motile cells/ml of medium) was determined at specific intervals using darkfield microscopy. In I. ricinus, the concentration of motile spirochetes increased significantly between the days 2 and 11 post inoculation (p.i.) with both SGE andMGE compared to control (C). In D. reticulatus, a significant increase in concentration of motile spirochetes was only detected with SGE (compared to control) on the day 5 p.i., while a marked decrease in concentration of motile spirochetes was observedon day 9 p.i. due to MGE effect, and on day 12 p.i. due to the effect of both extracts compared to control. The effect of SGE and MGE on the growth of B. garinii in vitro differed between the two ticks species tested: while the extracts

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia parasitologica

ISSN

0015-5683

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

159-160

Kód UT WoS článku

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EID výsledku v databázi Scopus

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