Izolace, kultivace a identifikace genospecies Borrelia burgdorferi z klíšťat Ixodes ricinus z města Brna, Česká republika

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F07%3A00089724" target="_blank" >RIV/68081766:_____/07:00089724 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation, cultivation and identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from the city of Brno, Czech Republic

Popis výsledku v původním jazyce

A total of 305 ticks (21 larvae, 243 nymphs, 41 adults) were collected by flagging vegetation in suburban woods of Pisarky Park (Brno) from July to October 2002. The midgut of each tick was dissected out and transferred into BSK-H medium. After cultivation, specimens were examined by DFM for the presence of borreliae. Out of 305 samples, 45 were (14.8%) positive. PCR test revealed 37 (12.1%) samples positive for B. burgdorferi s.l. DNA. PCR-RFLP analysis revealed 14 strains of B. afzelii (37.8%), 15 strains of B. garinii (40.5%) and 2 strains of B. burgdorferi sensu stricto (5.4%). Four samples (10.8%) showed a mixed infection of these genospecies. Two samples produced atypical RFLP pattern which were detected by sequence analysis as B. valaisiana (5.4%). Isolation attempts resulted in 21 strains (including two strains of B. valaisiana). The results show the diversity of B. burgdorferi s.l. in tick population and refer the risk of infection by pathogenic borreliae in Brno surroundings.

Název v anglickém jazyce

Isolation, cultivation and identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from the city of Brno, Czech Republic

Popis výsledku anglicky

A total of 305 ticks (21 larvae, 243 nymphs, 41 adults) were collected by flagging vegetation in suburban woods of Pisarky Park (Brno) from July to October 2002. The midgut of each tick was dissected out and transferred into BSK-H medium. After cultivation, specimens were examined by DFM for the presence of borreliae. Out of 305 samples, 45 were (14.8%) positive. PCR test revealed 37 (12.1%) samples positive for B. burgdorferi s.l. DNA. PCR-RFLP analysis revealed 14 strains of B. afzelii (37.8%), 15 strains of B. garinii (40.5%) and 2 strains of B. burgdorferi sensu stricto (5.4%). Four samples (10.8%) showed a mixed infection of these genospecies. Two samples produced atypical RFLP pattern which were detected by sequence analysis as B. valaisiana (5.4%). Isolation attempts resulted in 21 strains (including two strains of B. valaisiana). The results show the diversity of B. burgdorferi s.l. in tick population and refer the risk of infection by pathogenic borreliae in Brno surroundings.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Annals of Agricultural and Environmental Medicine

ISSN

1232-1966

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

5

Strana od-do

75-79

Kód UT WoS článku

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EID výsledku v databázi Scopus

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