Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F12%3A00379001" target="_blank" >RIV/68081766:_____/12:00379001 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.molbiopara.2012.07.002" target="_blank" >http://dx.doi.org/10.1016/j.molbiopara.2012.07.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molbiopara.2012.07.002" target="_blank" >10.1016/j.molbiopara.2012.07.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach

Popis výsledku v původním jazyce

Syphacia obvelata is a common gastro-intestinal parasitic nematode of the house mouse (Mus musculus), a prime model rodent species. Investigations of the genetic structure, variability of parasite populations and other biological aspects of this host?parasite system are limited due to the lack of genetic resources for S. obvelata. To fill this gap, we developed a set of microsatellite markers for S. obvelata, using a 454 pyrosequencing approach. We designed three multiplex panels allowing genotyping of10 polymorphic loci and scrutinized them on 42 samples from two different regions inhabited by two different house mouse subspecies (Mus musculus musculus and M. m. domesticus). The numbers of alleles ranged from 2 to 6 with mean observed heterozygosities 0.1476 and 0.2095 for domesticus and musculus worms, respectively. The described markers will facilitate further studies on population biology and co-evolution of this host?parasite system.

Název v anglickém jazyce

Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach

Popis výsledku anglicky

Syphacia obvelata is a common gastro-intestinal parasitic nematode of the house mouse (Mus musculus), a prime model rodent species. Investigations of the genetic structure, variability of parasite populations and other biological aspects of this host?parasite system are limited due to the lack of genetic resources for S. obvelata. To fill this gap, we developed a set of microsatellite markers for S. obvelata, using a 454 pyrosequencing approach. We designed three multiplex panels allowing genotyping of10 polymorphic loci and scrutinized them on 42 samples from two different regions inhabited by two different house mouse subspecies (Mus musculus musculus and M. m. domesticus). The numbers of alleles ranged from 2 to 6 with mean observed heterozygosities 0.1476 and 0.2095 for domesticus and musculus worms, respectively. The described markers will facilitate further studies on population biology and co-evolution of this host?parasite system.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Biochemical Parasitology

ISSN

0166-6851

e-ISSN

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Svazek periodika

185

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

154-156

Kód UT WoS článku

000310389700011

EID výsledku v databázi Scopus

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