Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00564481" target="_blank" >RIV/68081766:_____/22:00564481 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/22:00127773

Výsledek na webu

<a href="http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long" target="_blank" >http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1101/pdb.prot107739" target="_blank" >10.1101/pdb.prot107739</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)

Popis výsledku v původním jazyce

Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation.

Název v anglickém jazyce

Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)

Popis výsledku anglicky

Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10613 - Zoology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cold Spring Harbor protocols

ISSN

1940-3402

e-ISSN

1559-6095

Svazek periodika

2022

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

574-583

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85141888652