Heterosis versus breakdown in cyprinid hybrids associated with SVCV infection revealed by transcriptome profile analysis of head kidney

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F24%3A00575920" target="_blank" >RIV/68081766:_____/24:00575920 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00135237

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0044848623008578?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0044848623008578?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2023.740083" target="_blank" >10.1016/j.aquaculture.2023.740083</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Heterosis versus breakdown in cyprinid hybrids associated with SVCV infection revealed by transcriptome profile analysis of head kidney

Popis výsledku v původním jazyce

Whilst the hybrids of F1 generations usually experience heterosis for fitness-related traits (including the resistance to parasites), post-F1 generations, due to Dobzhansky–Muller genetic incompatibilities, express numerous disadvantageous traits (including susceptibility to parasites). Genetic disruption in hybrids may also result from the broken system of cyto-nuclear coadaptation. Maternal backcrosses (having parents with the same mtDNA) and paternal backcrosses (having parents with different mtDNA) have the same nuclear genetic compositions, but differ in cytoplasmic genetic elements, affecting their viability and survival. Spring viraemia of the carp virus (SVCV), a disease with a serious economic impact in aquacultures, affects almost exclusively cyprinids, primarily common carp (Cyprinus carpio), and causes high mortality, whilst gibel carp (Carassius gibelio) is a less susceptible species. Our study was focused on the transcriptome profile analysis of head kidney to reveal differential gene expression in highly susceptible common carp, weakly susceptible gibel carp, and hybrid lines, hypothetizing that the patterns of differential gene expression will reflect hybrid heterosis in F1 generations and hybrid breakdown in backcrosses and F2 generations. We expected the differences in differential gene expression between maternal and paternal backcrosses to be in line with the hypothesis of broken cyto-nuclear coadaptation. The pattern of differential gene expression revealed from the transcriptomic analysis of head kidney was linked to the resistance or susceptibility to SVCV infections in two pure species, C. gibelio and C. carpio, and their various generations of inter-specific hybrids. We evidenced hybrid heterosis for F1 generations of hybrids, mostly reflected by similarity in the down-regulation of differentially expressed genes classified within the viral infection disease category. High hybrid breakdown was documented throughout the transcriptomic analysis for paternal backcross generations and F2 generation of hybrids, confirming the broken system of cyto-nuclear coadaptation in those post-F1 generations. This evidence may support the role of intrinsic selection acting against inter-specific hybridization in freshwater fish.

Název v anglickém jazyce

Heterosis versus breakdown in cyprinid hybrids associated with SVCV infection revealed by transcriptome profile analysis of head kidney

Popis výsledku anglicky

Whilst the hybrids of F1 generations usually experience heterosis for fitness-related traits (including the resistance to parasites), post-F1 generations, due to Dobzhansky–Muller genetic incompatibilities, express numerous disadvantageous traits (including susceptibility to parasites). Genetic disruption in hybrids may also result from the broken system of cyto-nuclear coadaptation. Maternal backcrosses (having parents with the same mtDNA) and paternal backcrosses (having parents with different mtDNA) have the same nuclear genetic compositions, but differ in cytoplasmic genetic elements, affecting their viability and survival. Spring viraemia of the carp virus (SVCV), a disease with a serious economic impact in aquacultures, affects almost exclusively cyprinids, primarily common carp (Cyprinus carpio), and causes high mortality, whilst gibel carp (Carassius gibelio) is a less susceptible species. Our study was focused on the transcriptome profile analysis of head kidney to reveal differential gene expression in highly susceptible common carp, weakly susceptible gibel carp, and hybrid lines, hypothetizing that the patterns of differential gene expression will reflect hybrid heterosis in F1 generations and hybrid breakdown in backcrosses and F2 generations. We expected the differences in differential gene expression between maternal and paternal backcrosses to be in line with the hypothesis of broken cyto-nuclear coadaptation. The pattern of differential gene expression revealed from the transcriptomic analysis of head kidney was linked to the resistance or susceptibility to SVCV infections in two pure species, C. gibelio and C. carpio, and their various generations of inter-specific hybrids. We evidenced hybrid heterosis for F1 generations of hybrids, mostly reflected by similarity in the down-regulation of differentially expressed genes classified within the viral infection disease category. High hybrid breakdown was documented throughout the transcriptomic analysis for paternal backcross generations and F2 generation of hybrids, confirming the broken system of cyto-nuclear coadaptation in those post-F1 generations. This evidence may support the role of intrinsic selection acting against inter-specific hybridization in freshwater fish.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/GA19-10088S" target="_blank" >GA19-10088S: Genetická disrupce u mezidruhových hybridů kaprovitých ryb: její dopad na kondici-asociované znaky, imunitu a parazitární zátěž</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Svazek periodika

578

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

740083

Kód UT WoS článku

001081360000001

EID výsledku v databázi Scopus

2-s2.0-85171835777