The Cytotoxicity of TiO2 Nanomaterials as a Function of their Physico-Chemical Properties - a Generation of Data for Computational Modeling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F15%3A00459252" target="_blank" >RIV/68378041:_____/15:00459252 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Cytotoxicity of TiO2 Nanomaterials as a Function of their Physico-Chemical Properties - a Generation of Data for Computational Modeling

Popis výsledku v původním jazyce

Nanomaterials (NM) have become crucial for many applications promising significant improvement in various aspects of quality of life. The indisputable benefits of NM, however, might be discredited by concerns regarding the potential adverse health effects arising from unique properties related to the nanosize. The safety assessment of NM is complicated by the fact that conventional methods are insufficient for evaluating their toxicity. Although new methods adapted for NM are being extensively introduced by toxicologists, the lack of standardization causes inconsistency in existing toxicity data, thus making it impossible to draw general conclusions about NM toxicity. QualityNano is a current European FP7 project aimed at developing and implementing best practice and quality in nanosafety assessment. As one of the participating laboratories, we evaluated the cytotoxic potential of two reference NM (non-cytotoxic 40 nm carboxy-functionalized and cytotoxic 50 nm amino-functionalized polystyrene nanoparticles) in the lung adenocarcinoma A549 cell line using the QualityNano Standard Operation Procedure for the MTS Cell Viability Assay. The results of two performers showed good intra-laboratory concordance and the obtained data were provided for inter-laboratory variation assessment which is currently in progress within the QualityNano Network. The same protocol was used to evaluate the cytotoxicity of the reference NM sample in the immortalized bronchial epithelial BEAS-2B cell line. As expected, BEAS-2B cells were more susceptible than tumor A549 cells. Finally, two other methods were employed to investigate concordance among various cytotoxicity assays. Both the metabolic activity-based WST-1 assay and the LDH membrane integrity assay have proven to be relevant for studying NM cytotoxicity.

Název v anglickém jazyce

The Cytotoxicity of TiO2 Nanomaterials as a Function of their Physico-Chemical Properties - a Generation of Data for Computational Modeling

Popis výsledku anglicky

Nanomaterials (NM) have become crucial for many applications promising significant improvement in various aspects of quality of life. The indisputable benefits of NM, however, might be discredited by concerns regarding the potential adverse health effects arising from unique properties related to the nanosize. The safety assessment of NM is complicated by the fact that conventional methods are insufficient for evaluating their toxicity. Although new methods adapted for NM are being extensively introduced by toxicologists, the lack of standardization causes inconsistency in existing toxicity data, thus making it impossible to draw general conclusions about NM toxicity. QualityNano is a current European FP7 project aimed at developing and implementing best practice and quality in nanosafety assessment. As one of the participating laboratories, we evaluated the cytotoxic potential of two reference NM (non-cytotoxic 40 nm carboxy-functionalized and cytotoxic 50 nm amino-functionalized polystyrene nanoparticles) in the lung adenocarcinoma A549 cell line using the QualityNano Standard Operation Procedure for the MTS Cell Viability Assay. The results of two performers showed good intra-laboratory concordance and the obtained data were provided for inter-laboratory variation assessment which is currently in progress within the QualityNano Network. The same protocol was used to evaluate the cytotoxicity of the reference NM sample in the immortalized bronchial epithelial BEAS-2B cell line. As expected, BEAS-2B cells were more susceptible than tumor A549 cells. Finally, two other methods were employed to investigate concordance among various cytotoxicity assays. Both the metabolic activity-based WST-1 assay and the LDH membrane integrity assay have proven to be relevant for studying NM cytotoxicity.

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LO1508" target="_blank" >LO1508: Genomika a proteomika při studiu mechanismů biologických účinků vyráběných nanočástic</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

From Functionalized Nanostructures Towards Engineered Macrostructures

ISBN

978-80-88113-20-1

Počet stran výsledku

14

Strana od-do

35-48

Počet stran knihy

176

Název nakladatele

Epika

Místo vydání

Jindřichův Hradec

Kód UT WoS kapitoly

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