Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00451032" target="_blank" >RIV/68378041:_____/16:00451032 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S000527281500211X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S000527281500211X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2015.10.004" target="_blank" >10.1016/j.bbabio.2015.10.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function

Popis výsledku v původním jazyce

In yeast, phosphatidylglycerol (PG) is a minor phospholipid under standard conditions it can be utilized for cardiolipin (CL) biosynthesis by CL synthase, Crd1p, or alternatively degraded by the phospholipase Pgc1p. The Saccharomyces cerevisiae deletion mutants crd1 Delta and pgc1 Delta both accumulate PG. Based on analyses of the phospholipid content of pgc1 Delta, and crd1 Delta yeast, we revealed that in yeast mitochondria, two separate pools of PG are present, which differ in their fatty acid composition and accessibility for Pgcl p-catalyzed degradation. In contrast to CL-deficient crd1 Delta yeast, the pgc1 Delta mutant contains normal levels of CL. This makes the pgc1 Delta strain a suitable model to study the effect of accumulation of PG per se. Using fluorescence microscopy, we show that accumulation of PG with normal levels of CL resulted in increased fragmentation of mitochondria, while in the absence of CL, accumulation of PG led to the formation of large mitochondrial sheets. We also show that pgc1 Delta mitochondria exhibited increased respiration rates due to increased activity of cytochrome c oxidase. Taken together, our results indicate that not only a lack of anionic phospholipids, but also excess PG, or unbalanced ratios of anionic phospholipids in mitochondrial membranes, have harmful consequences on mitochondrial morphology and function. (C) 2015 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function

Popis výsledku anglicky

In yeast, phosphatidylglycerol (PG) is a minor phospholipid under standard conditions it can be utilized for cardiolipin (CL) biosynthesis by CL synthase, Crd1p, or alternatively degraded by the phospholipase Pgc1p. The Saccharomyces cerevisiae deletion mutants crd1 Delta and pgc1 Delta both accumulate PG. Based on analyses of the phospholipid content of pgc1 Delta, and crd1 Delta yeast, we revealed that in yeast mitochondria, two separate pools of PG are present, which differ in their fatty acid composition and accessibility for Pgcl p-catalyzed degradation. In contrast to CL-deficient crd1 Delta yeast, the pgc1 Delta mutant contains normal levels of CL. This makes the pgc1 Delta strain a suitable model to study the effect of accumulation of PG per se. Using fluorescence microscopy, we show that accumulation of PG with normal levels of CL resulted in increased fragmentation of mitochondria, while in the absence of CL, accumulation of PG led to the formation of large mitochondrial sheets. We also show that pgc1 Delta mitochondria exhibited increased respiration rates due to increased activity of cytochrome c oxidase. Taken together, our results indicate that not only a lack of anionic phospholipids, but also excess PG, or unbalanced ratios of anionic phospholipids in mitochondrial membranes, have harmful consequences on mitochondrial morphology and function. (C) 2015 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/GA15-10641S" target="_blank" >GA15-10641S: Specifické mikrodomény plasmatické membrány v regulaci stárnutí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Bioenergetics

ISSN

0005-2728

e-ISSN

—

Svazek periodika

1857

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

34-45

Kód UT WoS článku

000366771700005

EID výsledku v databázi Scopus

2-s2.0-84945917809