DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00469664" target="_blank" >RIV/68378041:_____/17:00469664 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21220/17:00303481 RIV/00216208:11310/17:10369600 RIV/00027162:_____/17:N0000047

Výsledek na webu

<a href="http://dx.doi.org/10.1111/bcpt.12693" target="_blank" >http://dx.doi.org/10.1111/bcpt.12693</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/bcpt.12693" target="_blank" >10.1111/bcpt.12693</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Popis výsledku v původním jazyce

Internal combustion engine emissions belong among the major anthropogenic sources of air pollution in urban areas. According to the International Agency for Research on Cancer, there is sufficient evidence of the carcinogenicity of diesel exhaust in human beings. Although alternative fuels, mainly biodiesel, have recently become popular, little is still known about the genotoxicity of emissions from these fuels. We analysed DNA damage expressed as the frequency of micronuclei (MN) in human bronchial epithelial cells (BEAS-2B), induced by extractable organic matter (EOM, tested concentrations: 1, 10 and 25 mu g/ml) obtained from particle emissions from various blends of biodiesel with diesel fuels (including neat diesel fuel (B0), a blend of 70% B0 and 30% biodiesel (B30) and neat biodiesel (B100)). We also tested the effect of selected diesel exhaust organic/genotoxic components [benzo[a] pyrene (B[a] P) concentrations: 25, 100 and 200 mu M, 1-nitropyrene (1-NP) concentrations: 1, 5 and 10 mu M, 3-nitrobenzanthrone (3-NBA) concentrations: 1, 5 and 50 mu M]. The cells were treated with the compounds for 28 and 48 hr. Our results showed that most of the tested compounds (except for the 25 mu M B[a] P, 28-hr treatment) significantly increased MN frequency. The genotoxicity of EOMs from the engine emissions of diesel and biodiesel engines was comparable. Both nitro-PAH compounds demonstrated higher genotoxic potential in comparison with B[a] P. Considering our results and due to increasing popularity of alternative fuels, it is prudent that the potential genotoxic effects of various fuels are investigated across engine technologies and operating conditions in a relevant model system.

Název v anglickém jazyce

DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Popis výsledku anglicky

Internal combustion engine emissions belong among the major anthropogenic sources of air pollution in urban areas. According to the International Agency for Research on Cancer, there is sufficient evidence of the carcinogenicity of diesel exhaust in human beings. Although alternative fuels, mainly biodiesel, have recently become popular, little is still known about the genotoxicity of emissions from these fuels. We analysed DNA damage expressed as the frequency of micronuclei (MN) in human bronchial epithelial cells (BEAS-2B), induced by extractable organic matter (EOM, tested concentrations: 1, 10 and 25 mu g/ml) obtained from particle emissions from various blends of biodiesel with diesel fuels (including neat diesel fuel (B0), a blend of 70% B0 and 30% biodiesel (B30) and neat biodiesel (B100)). We also tested the effect of selected diesel exhaust organic/genotoxic components [benzo[a] pyrene (B[a] P) concentrations: 25, 100 and 200 mu M, 1-nitropyrene (1-NP) concentrations: 1, 5 and 10 mu M, 3-nitrobenzanthrone (3-NBA) concentrations: 1, 5 and 50 mu M]. The cells were treated with the compounds for 28 and 48 hr. Our results showed that most of the tested compounds (except for the 25 mu M B[a] P, 28-hr treatment) significantly increased MN frequency. The genotoxicity of EOMs from the engine emissions of diesel and biodiesel engines was comparable. Both nitro-PAH compounds demonstrated higher genotoxic potential in comparison with B[a] P. Considering our results and due to increasing popularity of alternative fuels, it is prudent that the potential genotoxic effects of various fuels are investigated across engine technologies and operating conditions in a relevant model system.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Basic & Clinical Pharmacology & Toxicology

ISSN

1742-7835

e-ISSN

—

Svazek periodika

121

Číslo periodika v rámci svazku

SI

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

102-108

Kód UT WoS článku

000411036200012

EID výsledku v databázi Scopus

2-s2.0-85008413077