Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F18%3A00477248" target="_blank" >RIV/68378041:_____/18:00477248 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/18:00317978 RIV/68407700:21720/18:00317978 RIV/00216208:11130/18:10375268 RIV/00216208:11310/18:10375268

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/09537104.2017.1316838" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/09537104.2017.1316838</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/09537104.2017.1316838" target="_blank" >10.1080/09537104.2017.1316838</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers

Popis výsledku v původním jazyce

Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum - FBS replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells - fibroblasts, melanocytes, and keratinocytes were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% - v/v platelet lysate was sufficient to supplement 10% - v/v FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes, however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.

Název v anglickém jazyce

Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers

Popis výsledku anglicky

Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum - FBS replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells - fibroblasts, melanocytes, and keratinocytes were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% - v/v platelet lysate was sufficient to supplement 10% - v/v FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes, however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Platelets

ISSN

0953-7104

e-ISSN

—

Svazek periodika

29

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

395-405

Kód UT WoS článku

000430943700009

EID výsledku v databázi Scopus

2-s2.0-85021296699