Platelet Lysate as a Serum Replacement for Skin Cell Culture on Biomimetic PCL Nanofibers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00317978" target="_blank" >RIV/68407700:21460/18:00317978 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68407700:21720/18:00317978 RIV/00216208:11130/18:10375268 RIV/00216208:11310/18:10375268 RIV/68378041:_____/18:00477248 RIV/62157124:16170/18:43878394
Výsledek na webu
<a href="http://dx.doi.org/10.1080/09537104.2017.1316838" target="_blank" >http://dx.doi.org/10.1080/09537104.2017.1316838</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09537104.2017.1316838" target="_blank" >10.1080/09537104.2017.1316838</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Platelet Lysate as a Serum Replacement for Skin Cell Culture on Biomimetic PCL Nanofibers
Popis výsledku v původním jazyce
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% (v/v) platelet lysate was sufficient to supplement 10% (v/v) FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes; however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.
Název v anglickém jazyce
Platelet Lysate as a Serum Replacement for Skin Cell Culture on Biomimetic PCL Nanofibers
Popis výsledku anglicky
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% (v/v) platelet lysate was sufficient to supplement 10% (v/v) FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes; however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30500 - Other medical sciences
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Platelets
ISSN
0953-7104
e-ISSN
1369-1635
Svazek periodika
29
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
395-405
Kód UT WoS článku
000430943700009
EID výsledku v databázi Scopus
2-s2.0-85021296699