The Transdifferentiation of Adipose Tissue-Derived Mesenchymal Stem Cells Info Neural-like Cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F18%3A00500075" target="_blank" >RIV/68378041:_____/18:00500075 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Transdifferentiation of Adipose Tissue-Derived Mesenchymal Stem Cells Info Neural-like Cells

Popis výsledku v původním jazyce

The process of transdifferentiation of mesenchymal stem cells into neuron-like phenotype is a challenging and promising approach in the treatment of neurological diseases. To achieve neuron-like differentiation, we used human adipose tissue-derived mesenchymal stem cells (MSCs), which underwent long-term cultivation in the presence of growth factors, brain tissue postnuclear supernatant, supernatant from phytohemaglutitin (PHA)-stimulated lymphocytes and electrostimulation under physiological conditions. The differentiated cells showed an increased relative gene expression of neuron-specific markers (light neurofilament (Nf-L), neural cadherin (NCAM), beta-III tubulin (Tubb3) and glutamic acid decarboxylase (GAD)) and the presence of medium neurofilament (Nf-M) and Tubb-3 proteins. However, subsequent electrophysiological measurement did not reveal sensitivity to neurotransmitters and no spontaneous postsynaptic currents were observed. In conclusion, despite the expression of some neural specific markers, the current protocol was unsuccessful in achieving the complete transdifferentiation of MSCs to neuron-like cells.

Název v anglickém jazyce

The Transdifferentiation of Adipose Tissue-Derived Mesenchymal Stem Cells Info Neural-like Cells

Popis výsledku anglicky

The process of transdifferentiation of mesenchymal stem cells into neuron-like phenotype is a challenging and promising approach in the treatment of neurological diseases. To achieve neuron-like differentiation, we used human adipose tissue-derived mesenchymal stem cells (MSCs), which underwent long-term cultivation in the presence of growth factors, brain tissue postnuclear supernatant, supernatant from phytohemaglutitin (PHA)-stimulated lymphocytes and electrostimulation under physiological conditions. The differentiated cells showed an increased relative gene expression of neuron-specific markers (light neurofilament (Nf-L), neural cadherin (NCAM), beta-III tubulin (Tubb3) and glutamic acid decarboxylase (GAD)) and the presence of medium neurofilament (Nf-M) and Tubb-3 proteins. However, subsequent electrophysiological measurement did not reveal sensitivity to neurotransmitters and no spontaneous postsynaptic currents were observed. In conclusion, despite the expression of some neural specific markers, the current protocol was unsuccessful in achieving the complete transdifferentiation of MSCs to neuron-like cells.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/LO1309" target="_blank" >LO1309: Buněčná terapie a tkáňové náhrady</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Nanostructures in Biomedicine: Their Regenerative and toxic properties

ISBN

978-80-906655-7-6

Počet stran výsledku

7

Strana od-do

92-98

Počet stran knihy

198

Název nakladatele

Proxima Tisk, s.r.o.

Místo vydání

Praha 3

Kód UT WoS kapitoly

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