Analysis of Ca2+ Signaling Mechanisms - Our Experience on the Intercellular Communication in Muscle Remodeling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518849" target="_blank" >RIV/68378041:_____/19:00518849 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/19:10396566 RIV/00216208:11140/19:10396566

Výsledek na webu

<a href="http://www.biomed.cas.cz/physiolres/pdf/2019/68_325.pdf" target="_blank" >http://www.biomed.cas.cz/physiolres/pdf/2019/68_325.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33549/physiolres.934082" target="_blank" >10.33549/physiolres.934082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of Ca2+ Signaling Mechanisms - Our Experience on the Intercellular Communication in Muscle Remodeling

Popis výsledku v původním jazyce

The aim of this study was to evaluate cell diversity by considering how Ca2+ signaling has been adapted in skeletal muscle cell function. We characterized single C2C12 myoblasts through intracellular Ca2+ signaling kinetics after exposure to specific drugs and calcium blockers using fast fluorescence microspectrofluorimetry followed by ATP effect analysis, which confirmed the expression of functional purinergic adenosine and P2 receptors. Further, we found that glutamate sensitivity of C2C12 cells was mediated by ionotropic glutamate receptors, on the other hand, most cells were responsive to cyclopiazonic acid, which inhibits the sarco-endoplasmic reticulum Ca2+-ATPase pump. These results suggest that C2C12 cells possess functional L- and P/Q-type voltage-operated Ca2+ channels, ryanodine receptors and functional sarcoplasmic reticulum Ca2+ stores (typical for muscle cells), adenosine and P2 purinergic receptors, as well as ionotropic glutamate receptors. The evaluation of intracellular Ca2+ signaling is a promising approach towards a better understanding and control of the physiopathological properties of myogenic cells that could be used as a predictive factor in the selection of optimal cells for scaffold recellularization or for tissue engineered constructs used in stem cell therapy.

Název v anglickém jazyce

Analysis of Ca2+ Signaling Mechanisms - Our Experience on the Intercellular Communication in Muscle Remodeling

Popis výsledku anglicky

The aim of this study was to evaluate cell diversity by considering how Ca2+ signaling has been adapted in skeletal muscle cell function. We characterized single C2C12 myoblasts through intracellular Ca2+ signaling kinetics after exposure to specific drugs and calcium blockers using fast fluorescence microspectrofluorimetry followed by ATP effect analysis, which confirmed the expression of functional purinergic adenosine and P2 receptors. Further, we found that glutamate sensitivity of C2C12 cells was mediated by ionotropic glutamate receptors, on the other hand, most cells were responsive to cyclopiazonic acid, which inhibits the sarco-endoplasmic reticulum Ca2+-ATPase pump. These results suggest that C2C12 cells possess functional L- and P/Q-type voltage-operated Ca2+ channels, ryanodine receptors and functional sarcoplasmic reticulum Ca2+ stores (typical for muscle cells), adenosine and P2 purinergic receptors, as well as ionotropic glutamate receptors. The evaluation of intracellular Ca2+ signaling is a promising approach towards a better understanding and control of the physiopathological properties of myogenic cells that could be used as a predictive factor in the selection of optimal cells for scaffold recellularization or for tissue engineered constructs used in stem cell therapy.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/GA15-09161S" target="_blank" >GA15-09161S: Remodelace svalu na podkladě extracelulární matrix osázené funkčně charakterizovanými buňkami.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

325-328

Kód UT WoS článku

000465948800019

EID výsledku v databázi Scopus

2-s2.0-85065487824