Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F24%3A00587538" target="_blank" >RIV/68378041:_____/24:00587538 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/24:10484997

Výsledek na webu

<a href="https://www.nature.com/articles/s41565-024-01711-4" target="_blank" >https://www.nature.com/articles/s41565-024-01711-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41565-024-01711-4" target="_blank" >10.1038/s41565-024-01711-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes

Popis výsledku v původním jazyce

Astrocytes are responsible for maintaining homoeostasis and cognitive functions through calcium signalling, a process that is altered in brain diseases. Current bioelectronic tools are designed to study neurons and are not suitable for controlling calcium signals in astrocytes. Here, we show that electrical stimulation of astrocytes using electrodes coated with graphene oxide and reduced graphene oxide induces respectively a slow response to calcium, mediated by external calcium influx, and a sharp one, exclusively due to calcium release from intracellular stores. Our results suggest that the different conductivities of the substrate influence the electric field at the cell–electrolyte or cell–material interfaces, favouring different signalling events in vitro and ex vivo. Patch-clamp, voltage-sensitive dye and calcium imaging data support the proposed model. In summary, we provide evidence of a simple tool to selectively control distinct calcium signals in brain astrocytes for straightforward investigations in neuroscience and bioelectronic medicine.

Název v anglickém jazyce

Graphene oxide electrodes enable electrical stimulation of distinct calcium signalling in brain astrocytes

Popis výsledku anglicky

Astrocytes are responsible for maintaining homoeostasis and cognitive functions through calcium signalling, a process that is altered in brain diseases. Current bioelectronic tools are designed to study neurons and are not suitable for controlling calcium signals in astrocytes. Here, we show that electrical stimulation of astrocytes using electrodes coated with graphene oxide and reduced graphene oxide induces respectively a slow response to calcium, mediated by external calcium influx, and a sharp one, exclusively due to calcium release from intracellular stores. Our results suggest that the different conductivities of the substrate influence the electric field at the cell–electrolyte or cell–material interfaces, favouring different signalling events in vitro and ex vivo. Patch-clamp, voltage-sensitive dye and calcium imaging data support the proposed model. In summary, we provide evidence of a simple tool to selectively control distinct calcium signals in brain astrocytes for straightforward investigations in neuroscience and bioelectronic medicine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Nanotechnology

ISSN

1748-3387

e-ISSN

1748-3395

Svazek periodika

19

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

1344-1353

Kód UT WoS článku

001268978700002

EID výsledku v databázi Scopus

2-s2.0-85198123860