Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00617182" target="_blank" >RIV/68378041:_____/25:00617182 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26310/26:0193562

Výsledek na webu

<a href="https://accscience.com/journal/IJB/articles/online_first/4158" target="_blank" >https://accscience.com/journal/IJB/articles/online_first/4158</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.36922/ijb.5175" target="_blank" >10.36922/ijb.5175</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells

Popis výsledku v původním jazyce

Bone tissue engineering requires scaffolds with three-dimensional (3D) structures that facilitate vascularization and new tissue growth. 3D printing, especially through fused deposition modeling (FDM), has emerged as an effective method for creating complex structures with high reproducibility. Early research in this area demonstrated the potential of poly(ε-caprolactone) (PCL) and poly(L-lactide) (PLLA) scaffolds for bone regeneration. Recently, polylactide (PLA) and polyhydroxyalkanoates (PHAs) have garnered attention for their biocompatibility and ability to support cell proliferation. Among PHAs, poly(3-hydroxybutyrate) (PHB) shows promise due to its intrinsic biocompatibility and resorbability, making it a candidate for FDM-based scaffold fabrication. In the presented study, we aim to develop and optimize a biocompatible PHB-based composite material for bone tissue engineering, incorporating PLA, hydroxyapatite, and the plasticizer Syncroflex 3114 to enhance mechanical properties and printability. This composite was processed into filaments for 3D printing and characterized through thermal, mechanical, and biological evaluations. Using a design of experiment approach, we investigated factors such as temperature performance, warping, degradation, and strength to determine the optimal composition for use in tissue engineering. Four optimal mixture compositions fulfilling the optimization criteria of having the most suitable properties for bone tissue engineering, namely the best printability and maximum mechanical properties, were obtained. The mixtures were optimized specifically for minimum warping coefficient (0.5), maximum flexural strength (66.9 MPa), maximum compression modulus (2.4 GPa), and maximum compression modulus (2.3 GPa) with a warping coefficient of no more than 1 at the same time. In conclusion, the study shows a new possible way to effectively develop and test 3D-printed PHB-based scaffolds with specifically optimized material properties.

Název v anglickém jazyce

Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells

Popis výsledku anglicky

Bone tissue engineering requires scaffolds with three-dimensional (3D) structures that facilitate vascularization and new tissue growth. 3D printing, especially through fused deposition modeling (FDM), has emerged as an effective method for creating complex structures with high reproducibility. Early research in this area demonstrated the potential of poly(ε-caprolactone) (PCL) and poly(L-lactide) (PLLA) scaffolds for bone regeneration. Recently, polylactide (PLA) and polyhydroxyalkanoates (PHAs) have garnered attention for their biocompatibility and ability to support cell proliferation. Among PHAs, poly(3-hydroxybutyrate) (PHB) shows promise due to its intrinsic biocompatibility and resorbability, making it a candidate for FDM-based scaffold fabrication. In the presented study, we aim to develop and optimize a biocompatible PHB-based composite material for bone tissue engineering, incorporating PLA, hydroxyapatite, and the plasticizer Syncroflex 3114 to enhance mechanical properties and printability. This composite was processed into filaments for 3D printing and characterized through thermal, mechanical, and biological evaluations. Using a design of experiment approach, we investigated factors such as temperature performance, warping, degradation, and strength to determine the optimal composition for use in tissue engineering. Four optimal mixture compositions fulfilling the optimization criteria of having the most suitable properties for bone tissue engineering, namely the best printability and maximum mechanical properties, were obtained. The mixtures were optimized specifically for minimum warping coefficient (0.5), maximum flexural strength (66.9 MPa), maximum compression modulus (2.4 GPa), and maximum compression modulus (2.3 GPa) with a warping coefficient of no more than 1 at the same time. In conclusion, the study shows a new possible way to effectively develop and test 3D-printed PHB-based scaffolds with specifically optimized material properties.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

<a href="/cs/project/EH22_008%2F0004562" target="_blank" >EH22_008/0004562: Excelentní výzkum v regenerativní medicíně</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Bioprinting

ISSN

2424-7723

e-ISSN

2424-8002

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SG - Singapurská republika

Počet stran výsledku

18

Strana od-do

400-417

Kód UT WoS článku

001501058400002

EID výsledku v databázi Scopus

2-s2.0-85218863490