Enhancing adipogenesis in Wharton’s jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00641458" target="_blank" >RIV/68378041:_____/25:00641458 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/25:00638929

Výsledek na webu

<a href="https://doi.org/10.1038/s41598-025-16867-9" target="_blank" >https://doi.org/10.1038/s41598-025-16867-9</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Enhancing adipogenesis in Wharton’s jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation

Popis výsledku v původním jazyce

Wharton’s Jelly multipotent mesenchymal stromal cells (WJ-MSCs) hold potential for regenerativenmedicine, particularly in soft tissue engineering. However, their adipogenic differentiation capacitynis inferior to adipose tissue-derived MSCs (AT-MSCs). This study aimed to optimize adipogenicndifferentiation for WJ-MSCs by leveraging insights from the comparative analysis of WJ- and ATMSCnlipidomic profiles. Lipidomic profiles of non-induced cells were compared, and adipogenicndifferentiation was induced with and without exogenous oleic or linoleic acid supplementation.nDifferentiation efficiency was determined based on lipid droplet formation, triglyceride (TG)ncontent quantification, and the expression of adipogenic markers. Significant differences in TGncomposition were observed, with WJ-MSCs showing higher levels of 52-carbon TGs and AT-MSCsnhaving more 56-carbon species. Both cell types had similar fatty acid (FA) profiles, with 18-carbonnFAs making up over 50%. Adding oleic acid to the differentiation medium significantly enhancednlipid droplet formation and upregulated adipogenic markers in WJ-MSCs, aligning their adipogenicncapacity more closely with AT-MSCs. In contrast, linoleic acid showed no significant benefits. Thenstudy underscores the critical role of the initial lipidomic profile in the adipogenic differentiation ofnMSCs. Supplementation with oleic acid represents a promising approach for improving adipogenicndifferentiation of WJ-MSCs and their utility in soft tissue engineering

Název v anglickém jazyce

Enhancing adipogenesis in Wharton’s jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation

Popis výsledku anglicky

Wharton’s Jelly multipotent mesenchymal stromal cells (WJ-MSCs) hold potential for regenerativenmedicine, particularly in soft tissue engineering. However, their adipogenic differentiation capacitynis inferior to adipose tissue-derived MSCs (AT-MSCs). This study aimed to optimize adipogenicndifferentiation for WJ-MSCs by leveraging insights from the comparative analysis of WJ- and ATMSCnlipidomic profiles. Lipidomic profiles of non-induced cells were compared, and adipogenicndifferentiation was induced with and without exogenous oleic or linoleic acid supplementation.nDifferentiation efficiency was determined based on lipid droplet formation, triglyceride (TG)ncontent quantification, and the expression of adipogenic markers. Significant differences in TGncomposition were observed, with WJ-MSCs showing higher levels of 52-carbon TGs and AT-MSCsnhaving more 56-carbon species. Both cell types had similar fatty acid (FA) profiles, with 18-carbonnFAs making up over 50%. Adding oleic acid to the differentiation medium significantly enhancednlipid droplet formation and upregulated adipogenic markers in WJ-MSCs, aligning their adipogenicncapacity more closely with AT-MSCs. In contrast, linoleic acid showed no significant benefits. Thenstudy underscores the critical role of the initial lipidomic profile in the adipogenic differentiation ofnMSCs. Supplementation with oleic acid represents a promising approach for improving adipogenicndifferentiation of WJ-MSCs and their utility in soft tissue engineering

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

30962

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—