Comparison of multipotent mesenchymal stromal cells from human adipose tissue and Wharton's jelly for soft tissue reconstruction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F20%3A00348844" target="_blank" >RIV/68407700:21460/20:00348844 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21720/20:00348844

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of multipotent mesenchymal stromal cells from human adipose tissue and Wharton's jelly for soft tissue reconstruction

Popis výsledku v původním jazyce

Adipose tissue engineering, based on the application of multipotent mesenchymal stromal cells (MSCs) represents a promising strategy to restore soft tissue defects. The source of MSCs for the application in adipose tissue engineering may determine the efficiency of reconstruction strategy. The capacity to differentiate towards adipogenic lineage is one of the criteria defining fibroblast-like cells to MSC. However, the efficiency of the differentiation depending on the source of cell isolation is not determined as a standard and comparative studies are needed to find the optimal source of MSC for adipose / soft tissue reconstruction. The aim of the study was to evaluate the potential of MSCs derived from adipose tissue (AT) and Wharton’s jelly (WJ) for adipose tissue engineering. We assessed the effect of the presence of serum in the adipogenic differentiation medium. Finally, we assessed the possibility of using established culture and differentiation conditions for the adipose tissue engineering in 3D collagen scaffolds.

Název v anglickém jazyce

Comparison of multipotent mesenchymal stromal cells from human adipose tissue and Wharton's jelly for soft tissue reconstruction

Popis výsledku anglicky

Adipose tissue engineering, based on the application of multipotent mesenchymal stromal cells (MSCs) represents a promising strategy to restore soft tissue defects. The source of MSCs for the application in adipose tissue engineering may determine the efficiency of reconstruction strategy. The capacity to differentiate towards adipogenic lineage is one of the criteria defining fibroblast-like cells to MSC. However, the efficiency of the differentiation depending on the source of cell isolation is not determined as a standard and comparative studies are needed to find the optimal source of MSC for adipose / soft tissue reconstruction. The aim of the study was to evaluate the potential of MSCs derived from adipose tissue (AT) and Wharton’s jelly (WJ) for adipose tissue engineering. We assessed the effect of the presence of serum in the adipogenic differentiation medium. Finally, we assessed the possibility of using established culture and differentiation conditions for the adipose tissue engineering in 3D collagen scaffolds.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů