Isolation of non-heprin-binding and heparin-binding proteins of boar prostate.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F02%3A23033121" target="_blank" >RIV/68378050:_____/02:23033121 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation of non-heprin-binding and heparin-binding proteins of boar prostate.

Popis výsledku v původním jazyce

Proteins of boar prostet secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding and heparin-binding protein fractions. These fractions were thwn subjected to RP HPLC. Elution profiles of fractions of prostatesecretion were compared with non-heparin-binding and heparin-binding protein fractions of seminal plasma. Besides, the isolated proteins were characterized by SDS-PAGE. In protein fractions of prostate secretion, PSP I, PSP II, AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the countrary, beta-microseminoprotein was identified in abundant amounts in prostate secretion. Some seminal plasma proteins originating mainly inseminal vesicles could also be secreted by the prostatic gland. Beta-microseminoprotein was found to be produced mainly by the prostate.

Název v anglickém jazyce

Isolation of non-heprin-binding and heparin-binding proteins of boar prostate.

Popis výsledku anglicky

Proteins of boar prostet secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding and heparin-binding protein fractions. These fractions were thwn subjected to RP HPLC. Elution profiles of fractions of prostatesecretion were compared with non-heparin-binding and heparin-binding protein fractions of seminal plasma. Besides, the isolated proteins were characterized by SDS-PAGE. In protein fractions of prostate secretion, PSP I, PSP II, AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the countrary, beta-microseminoprotein was identified in abundant amounts in prostate secretion. Some seminal plasma proteins originating mainly inseminal vesicles could also be secreted by the prostatic gland. Beta-microseminoprotein was found to be produced mainly by the prostate.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

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Svazek periodika

770

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

7

Strana od-do

137-143

Kód UT WoS článku

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EID výsledku v databázi Scopus

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