Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F03%3A23033091" target="_blank" >RIV/68378050:_____/03:23033091 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis.

Popis výsledku v původním jazyce

Video Rate Confocal Laser Scanning Microscope (VRCLSM) Odyssey (Noran, Middleton, WI, USA) at the highest spatial and temporal resolution of back scattered light (BSL) imaging enabled regular observation of fast intracellular motion (FIM) first revealedin living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motionand a known type of motion such as cell marginal ruffling compared with FIM. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysisof FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color coded images only.

Název v anglickém jazyce

Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis.

Popis výsledku anglicky

Video Rate Confocal Laser Scanning Microscope (VRCLSM) Odyssey (Noran, Middleton, WI, USA) at the highest spatial and temporal resolution of back scattered light (BSL) imaging enabled regular observation of fast intracellular motion (FIM) first revealedin living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motionand a known type of motion such as cell marginal ruffling compared with FIM. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysisof FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color coded images only.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA304%2F99%2F0368" target="_blank" >GA304/99/0368: Význam rychlých vnitrobuněčných motilitních reakcí živých buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

SCANNING

ISSN

0161-0457

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

230-239

Kód UT WoS článku

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EID výsledku v databázi Scopus

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