Receptor pro ptačí sarkomové a leukózové viry podskupiny C Tvc je příbuzný savčím butyrofilinům, členům nadrodiny imunoglobuilinů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00020887" target="_blank" >RIV/68378050:_____/05:00020887 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily

Popis výsledku v původním jazyce

We cloned the subgroup C ASLV receptor gene (tvc) and identified the Tvc receptor. The tvc gene was identified on a bacterial artificial chromosome containing a portion of chicken chromosome 28 that conferred susceptibility to ASLV(C) infection and encodes a 488-amino-acid protein most closely related to mammalian butyrophilins. We cloned cDNAs encoding Tvc that confer susceptibility to infection by subgroup C viruses in chicken cells resistant to ASLV(C) infection and in mammalian cells that do not normally express functional ASLV receptors. In addition, normally susceptible chicken DT40 cells were resistant to ASLV(C) infection after both tvc alleles were disrupted by homologous recombination. Tvc binds the ASLV(C) envelope glycoproteins with low-nanomolar affinity, similar to that of binding of Tva and Tvb with their envelope glycoproteins. We identified a mutation in the tvc gene in line L15 chickens that explains why this line is resistant to ASLV(C) infection.

Název v anglickém jazyce

The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily

Popis výsledku anglicky

We cloned the subgroup C ASLV receptor gene (tvc) and identified the Tvc receptor. The tvc gene was identified on a bacterial artificial chromosome containing a portion of chicken chromosome 28 that conferred susceptibility to ASLV(C) infection and encodes a 488-amino-acid protein most closely related to mammalian butyrophilins. We cloned cDNAs encoding Tvc that confer susceptibility to infection by subgroup C viruses in chicken cells resistant to ASLV(C) infection and in mammalian cells that do not normally express functional ASLV receptors. In addition, normally susceptible chicken DT40 cells were resistant to ASLV(C) infection after both tvc alleles were disrupted by homologous recombination. Tvc binds the ASLV(C) envelope glycoproteins with low-nanomolar affinity, similar to that of binding of Tva and Tvb with their envelope glycoproteins. We identified a mutation in the tvc gene in line L15 chickens that explains why this line is resistant to ASLV(C) infection.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA523%2F04%2F0489" target="_blank" >GA523/04/0489: Patogenní působení ptačích leukózových virů (ALV): molekulární a genetické aspekty</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Virology

ISSN

0022-538X

e-ISSN

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Svazek periodika

79

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

10408-10419

Kód UT WoS článku

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EID výsledku v databázi Scopus

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