Jednoaminokyselinová substituce v receptoru TvbS1 snižuje susceptibilitu k infekci ptačími sarkomovými a leukosovými viry podskupin B a D a podmiňuje resistenci k podskupině E in vitro a in vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00306812" target="_blank" >RIV/68378050:_____/08:00306812 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo

Popis výsledku v původním jazyce

ASLV family retroviruses contain 5 highly related envelope subgroups (A-E). Susceptibility or resistance of cells to infection by these ASLVs is given by 3 loci in chicken. The tvb gene encodes subgroup B, D, E ASLV receptors. Wild-type TvbS1 receptor confers susceptibility to subgroup B, D, E ASLVs. In this study the genetic defect underlying altered susceptibility of inbred chicken line M to infection by ASLV(B), (D), (E), C125S substitution in tvb-encoded TvbS1 receptor in this line, was identified.We show that this substitution significantly reduces susceptibility of line M cells to infection by ASLV(B) and (D) and virtually eliminates susceptibility to ASLV(E) infection in cultured cells and the incidence and growth of ASV-induced sarcomas in chickens. It also significantly reduces binding affinity of TvbS1 receptor for subgroup B, D, E ASLV envelope glycoproteins. These results are first demonstration of possible role of the Cys-rich domain 3 in the function of Tvb receptors.

Název v anglickém jazyce

A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo

Popis výsledku anglicky

ASLV family retroviruses contain 5 highly related envelope subgroups (A-E). Susceptibility or resistance of cells to infection by these ASLVs is given by 3 loci in chicken. The tvb gene encodes subgroup B, D, E ASLV receptors. Wild-type TvbS1 receptor confers susceptibility to subgroup B, D, E ASLVs. In this study the genetic defect underlying altered susceptibility of inbred chicken line M to infection by ASLV(B), (D), (E), C125S substitution in tvb-encoded TvbS1 receptor in this line, was identified.We show that this substitution significantly reduces susceptibility of line M cells to infection by ASLV(B) and (D) and virtually eliminates susceptibility to ASLV(E) infection in cultured cells and the incidence and growth of ASV-induced sarcomas in chickens. It also significantly reduces binding affinity of TvbS1 receptor for subgroup B, D, E ASLV envelope glycoproteins. These results are first demonstration of possible role of the Cys-rich domain 3 in the function of Tvb receptors.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Virology

ISSN

0022-538X

e-ISSN

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Svazek periodika

82

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000253481000006

EID výsledku v databázi Scopus

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