Společná lokalizace tetraspaninů CO-029 a CD151 s integriny u lidských adenokarcinomů slinivky: vliv na buněčnou motilitu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00021319" target="_blank" >RIV/68378050:_____/05:00021319 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Colocalization of the tetraspanins, CO-029 and CD151, with integrins in human pancreatic adenocarcinoma: impact on cell motility

Popis výsledku v původním jazyce

The majority of pancreatic and colorectal tumors expressed the a2, a3, a6, b1, and b4 integrins and the tetraspanins CD9, CD63, CD81, CD151, and CO-029. Expression of a6b4 and CO-029 was restricted to tumor cells, whereas a1, a2, a3, a6, b1, and CD9, CD81, CD151 were also expressed by the surrounding stroma. CD63, CD81, and b1 expression was observed at comparably high levels in healthy pancreatic tissue. al3b1 frequently colocalized and coimmunoprecipitated with CD9, CD81, and CD151, whereas a6b4 colocalized and coimmunoprecipitated mostly with CD151 and CO-029. Notably, protein kinase C activation strengthened only the colocalization of CD151 and CO-029 with b4 and was accompanied by internalization of the integrin-tetraspanin complex, decreased laminin 5 adhesion, and increased cell migration. CONCLUSION: Integrin a6b4 is selectively up-regulated in pancreatic and colorectal cancer. The association of a6b4 with CD151 and CO-029 correlates with increased tumor cell motility.

Název v anglickém jazyce

Colocalization of the tetraspanins, CO-029 and CD151, with integrins in human pancreatic adenocarcinoma: impact on cell motility

Popis výsledku anglicky

The majority of pancreatic and colorectal tumors expressed the a2, a3, a6, b1, and b4 integrins and the tetraspanins CD9, CD63, CD81, CD151, and CO-029. Expression of a6b4 and CO-029 was restricted to tumor cells, whereas a1, a2, a3, a6, b1, and CD9, CD81, CD151 were also expressed by the surrounding stroma. CD63, CD81, and b1 expression was observed at comparably high levels in healthy pancreatic tissue. al3b1 frequently colocalized and coimmunoprecipitated with CD9, CD81, and CD151, whereas a6b4 colocalized and coimmunoprecipitated mostly with CD151 and CO-029. Notably, protein kinase C activation strengthened only the colocalization of CD151 and CO-029 with b4 and was accompanied by internalization of the integrin-tetraspanin complex, decreased laminin 5 adhesion, and increased cell migration. CONCLUSION: Integrin a6b4 is selectively up-regulated in pancreatic and colorectal cancer. The association of a6b4 with CD151 and CO-029 correlates with increased tumor cell motility.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Cancer Research

ISSN

1078-0432

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

2840-2852

Kód UT WoS článku

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EID výsledku v databázi Scopus

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