Exprese beta-kateninu je v kolorektálních nádorových buňkách regulována PI-3 kinázou a butyrátem sodným

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00024289" target="_blank" >RIV/68378050:_____/05:00024289 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/06:00043549

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of beta-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells

Popis výsledku v původním jazyce

?-catenin is implicated in intercellular junctions and transcriptional co-activation. Here we examined regulation of the expression of ?-catenin in HT29 colorectal adenocarcinoma cells. Our results showed that inhibition of PI-3 kinase with wortmannin was accompanied by a considerably reduced expression of ?-catenin. This effect was overcome by butyrate and occured at the protein level, not at the level of mRNA. Moreover, NaBT significantly increased phosphorylation of the ribosomal protein S6, participating in translational control of gene expression. This was accompanied by the increased phosphorylation of p70 S6K and MAPKs, the effector proteins that are upstream of protein S6 in the distinct signaling pathways. These facts indicate that different signaling pathways may be involved in the regulation of ?-catenin synthesis. Modulation of ?-catenin expression by NaBT occured at the level of protein translation, suggesting that NaBT may act as a translational regulator.

Název v anglickém jazyce

Expression of beta-catenin is regulated by PI-3 kinase and sodium butyrate in colorectal cancer cells

Popis výsledku anglicky

?-catenin is implicated in intercellular junctions and transcriptional co-activation. Here we examined regulation of the expression of ?-catenin in HT29 colorectal adenocarcinoma cells. Our results showed that inhibition of PI-3 kinase with wortmannin was accompanied by a considerably reduced expression of ?-catenin. This effect was overcome by butyrate and occured at the protein level, not at the level of mRNA. Moreover, NaBT significantly increased phosphorylation of the ribosomal protein S6, participating in translational control of gene expression. This was accompanied by the increased phosphorylation of p70 S6K and MAPKs, the effector proteins that are upstream of protein S6 in the distinct signaling pathways. These facts indicate that different signaling pathways may be involved in the regulation of ?-catenin synthesis. Modulation of ?-catenin expression by NaBT occured at the level of protein translation, suggesting that NaBT may act as a translational regulator.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Medicine

ISSN

1107-3756

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

7

Strana od-do

69-75

Kód UT WoS článku

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EID výsledku v databázi Scopus

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