Různé formy rezistence hlodavců vůči vstupu prasečího endogenního retroviru podskupiny A do buňky

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F07%3A00306859" target="_blank" >RIV/68378050:_____/07:00306859 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species

Popis výsledku v původním jazyce

The risk of zoonotic infection by porcine endogenous retroviruses (PERV) has been highlighted in the context of pig-to-human xenotransplantation. A human-tropic PERV subgroup, PERV-A, can enter human cells through either of two homologous transmembrane proteins, huPAR-1 and huPAR-2. Here, we characterised human PARs and their homologues in the PERV-A resistant rodent species, mouse and rat (muPAR and ratPAR). Upon exogenous expression in PERV-A resistant cells, human and rat PARs, but not muPAR, conferred PERV-A sensitivity. Exogenously expressed ratPAR allows PERV-A infection with equivalent efficiency to that of huPAR-1. Endogenous ratPAR expression in rat cell lines appeared to be too low for PERV-A infection. In contrast, in mouse the PERV-A resistance is caused by the presence of Pro at position 109 in the second extracelular loop of muPAR, which affects the PERV-A Env binding to PAR molecules. Both rodent species are PERV-A resistant, however, on different basis.

Název v anglickém jazyce

Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species

Popis výsledku anglicky

The risk of zoonotic infection by porcine endogenous retroviruses (PERV) has been highlighted in the context of pig-to-human xenotransplantation. A human-tropic PERV subgroup, PERV-A, can enter human cells through either of two homologous transmembrane proteins, huPAR-1 and huPAR-2. Here, we characterised human PARs and their homologues in the PERV-A resistant rodent species, mouse and rat (muPAR and ratPAR). Upon exogenous expression in PERV-A resistant cells, human and rat PARs, but not muPAR, conferred PERV-A sensitivity. Exogenously expressed ratPAR allows PERV-A infection with equivalent efficiency to that of huPAR-1. Endogenous ratPAR expression in rat cell lines appeared to be too low for PERV-A infection. In contrast, in mouse the PERV-A resistance is caused by the presence of Pro at position 109 in the second extracelular loop of muPAR, which affects the PERV-A Env binding to PAR molecules. Both rodent species are PERV-A resistant, however, on different basis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Retrovirology

ISSN

1742-4690

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

000253349500001

EID výsledku v databázi Scopus

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