Imunoterapie minimální zbytkové choroby interleukinem 12 na myším modelu nádorů asociovaných s virem HPV 16: Indukce imunitní odpovědi, produkce cytokinů a kinetika subpopulací imunních buněk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00306790" target="_blank" >RIV/68378050:_____/08:00306790 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

IL-12 immunotherapy of minimal residual disease in murine models of HPV16-associated tumours: induction of immune responses, cytokine production and kinetics of immune cell subsets

Popis výsledku v původním jazyce

In this model, upregulation of cytokine production was detected, compared to the animals without tumours. But, no differences in Th1/Th2 polarization of the immune responses were observed. In the spleens of TC-1 (MHC class I+) but not of TC-1/A9 (MHC class I?) treated tumour-bearing animals, the cytotoxic CD8+ cells were found. In the spleens of TC-1/A9 but not of TC-1 tumour-treated animals, the NK activity measured as the lysis of NK-sensitive YAC-1 targets was detected. Downregulation of the CD4+ andCD8+ subpopulations were not restored after therapy. The percentage of CD25+/CD4+ T regulatory cells in lymph nodes remained unchanged. The chemotherapy led to upregulation of immunosuppressive immature myeloid Gr-1+/CD11b+ (IMC) cells in the spleens oftreated animals. The accumulation of IMC was significantly decreased after subsequent IL-12 immunotherapy. That elimination may be responsible for the improvement of antitumour responses after adjuvant IL-12 vaccination.

Název v anglickém jazyce

IL-12 immunotherapy of minimal residual disease in murine models of HPV16-associated tumours: induction of immune responses, cytokine production and kinetics of immune cell subsets

Popis výsledku anglicky

In this model, upregulation of cytokine production was detected, compared to the animals without tumours. But, no differences in Th1/Th2 polarization of the immune responses were observed. In the spleens of TC-1 (MHC class I+) but not of TC-1/A9 (MHC class I?) treated tumour-bearing animals, the cytotoxic CD8+ cells were found. In the spleens of TC-1/A9 but not of TC-1 tumour-treated animals, the NK activity measured as the lysis of NK-sensitive YAC-1 targets was detected. Downregulation of the CD4+ andCD8+ subpopulations were not restored after therapy. The percentage of CD25+/CD4+ T regulatory cells in lymph nodes remained unchanged. The chemotherapy led to upregulation of immunosuppressive immature myeloid Gr-1+/CD11b+ (IMC) cells in the spleens oftreated animals. The accumulation of IMC was significantly decreased after subsequent IL-12 immunotherapy. That elimination may be responsible for the improvement of antitumour responses after adjuvant IL-12 vaccination.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F06%2F0774" target="_blank" >GA301/06/0774: Úloha NK buněk, Th buněk a buněk infiltrujících nádor při chemoterapii a genové léčbě MHC I deficientních nádorů asociovaných s virem HPV 16</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Oncology

ISSN

1019-6439

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000252620900025

EID výsledku v databázi Scopus

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