Wnt produkující krysí embryonální fibroblasty potlačují Apo2L/TRAIL indukovanou apoptózu lidských leukemických buněk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F08%3A00306874" target="_blank" >RIV/68378050:_____/08:00306874 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells

Popis výsledku v původním jazyce

The Wnt signaling enhances proliferation and maintenance of hematopoietic cells. In contrary, the cytotoxic ligand Apo2L/TRAIL induces apoptosis of transformed cells. We observed that pre-cultivation of pre-B leukemia cell lines (KM3, REH) on Wnt1- or Wnt3a-expressing Rat2 fibroblasts efficiently suppressed their Apo2L/TRAIL -induced apoptosis. This suppression occurs at the early stages of the Apo2L/TRAIL apoptotic signaling and is only partially dependent on the Wnt pathway alone. Anti-apoptotic Rat2Wnt1 signaling was significantly hampered by the inhibition of MEK1/MEK2 or NFkB pathways in pre-B acceptor cells. Our results implicate the existence of an additional signaling pathway in Wnt1-transfected fibroblasts that in conjunction with the Wnt-triggered cascade of cellular events suppresses Apo2L/TRAIL-induced apoptosis of pre-B cells. Such composed antiapoptotic signaling could contribute to pre-B leukemias survival.

Název v anglickém jazyce

Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells

Popis výsledku anglicky

The Wnt signaling enhances proliferation and maintenance of hematopoietic cells. In contrary, the cytotoxic ligand Apo2L/TRAIL induces apoptosis of transformed cells. We observed that pre-cultivation of pre-B leukemia cell lines (KM3, REH) on Wnt1- or Wnt3a-expressing Rat2 fibroblasts efficiently suppressed their Apo2L/TRAIL -induced apoptosis. This suppression occurs at the early stages of the Apo2L/TRAIL apoptotic signaling and is only partially dependent on the Wnt pathway alone. Anti-apoptotic Rat2Wnt1 signaling was significantly hampered by the inhibition of MEK1/MEK2 or NFkB pathways in pre-B acceptor cells. Our results implicate the existence of an additional signaling pathway in Wnt1-transfected fibroblasts that in conjunction with the Wnt-triggered cascade of cellular events suppresses Apo2L/TRAIL-induced apoptosis of pre-B cells. Such composed antiapoptotic signaling could contribute to pre-B leukemias survival.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Apoptosis

ISSN

1360-8185

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

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Kód UT WoS článku

000254302400010

EID výsledku v databázi Scopus

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